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Temporal and lineage-specific control of T cell receptor alpha/delta gene rearrangement by T cell receptor alpha and delta enhancers.

Publication ,  Journal Article
Lauzurica, P; Krangel, MS
Published in: J Exp Med
June 1, 1994

To analyze the regulation of gene rearrangement at the T cell receptor (TCR) alpha/delta locus during T cell development, we generated transgenic mice carrying a human TCR delta gene minilocus. We previously showed that the presence of the TCR delta enhancer (E delta) within the J delta 3-C delta intron was required to activate a specific step (V-D to J) of transgene rearrangement, and that rearrangement was activated equivalently in the precursors of alpha beta and gamma delta T cells. To further explore the role of transcriptional enhancers in establishing the developmental pattern of gene rearrangement at the TCR alpha/delta locus, we substituted the TCR alpha enhancer (E alpha) in place of E delta within the transgenic minilocus. We found that V-D-J rearrangement of the E alpha+ minilocus was restricted to the alpha beta T cell subset. Further, we found that although V-D-J rearrangement of the E delta+ minilocus was initiated in the fetal thymus by day 14.5, V-D-J rearrangement of the E alpha+ minilocus did not occur until fetal day 16.5. Finally, whereas V-D-J rearrangement of the E delta+ minilocus is essentially completed within the triple negative population of postnatal thymocytes, V-D-J rearrangement of the E alpha+ minilocus is only initiated late within this population. Since the properties of minilocus rearrangement under the control of E delta and E alpha parallel the properties of V delta-D delta-J delta and V alpha-J alpha rearrangement at the endogenous TCR alpha/delta locus, we conclude that these enhancers play an important role in orchestrating the developmental program of rearrangements at this locus.

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Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

June 1, 1994

Volume

179

Issue

6

Start / End Page

1913 / 1921

Location

United States

Related Subject Headings

  • Thymus Gland
  • T-Lymphocytes
  • Receptors, Antigen, T-Cell, gamma-delta
  • Polymerase Chain Reaction
  • Organ Specificity
  • Mice, Transgenic
  • Mice, Inbred Strains
  • Mice, Inbred C57BL
  • Mice
  • Male
 

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Lauzurica, P., & Krangel, M. S. (1994). Temporal and lineage-specific control of T cell receptor alpha/delta gene rearrangement by T cell receptor alpha and delta enhancers. J Exp Med, 179(6), 1913–1921. https://doi.org/10.1084/jem.179.6.1913
Lauzurica, P., and M. S. Krangel. “Temporal and lineage-specific control of T cell receptor alpha/delta gene rearrangement by T cell receptor alpha and delta enhancers.J Exp Med 179, no. 6 (June 1, 1994): 1913–21. https://doi.org/10.1084/jem.179.6.1913.
Lauzurica, P., and M. S. Krangel. “Temporal and lineage-specific control of T cell receptor alpha/delta gene rearrangement by T cell receptor alpha and delta enhancers.J Exp Med, vol. 179, no. 6, June 1994, pp. 1913–21. Pubmed, doi:10.1084/jem.179.6.1913.

Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

June 1, 1994

Volume

179

Issue

6

Start / End Page

1913 / 1921

Location

United States

Related Subject Headings

  • Thymus Gland
  • T-Lymphocytes
  • Receptors, Antigen, T-Cell, gamma-delta
  • Polymerase Chain Reaction
  • Organ Specificity
  • Mice, Transgenic
  • Mice, Inbred Strains
  • Mice, Inbred C57BL
  • Mice
  • Male