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Development of [3H]nicotine binding sites in brain regions of rats exposed to nicotine prenatally via maternal injections or infusions.

Publication ,  Journal Article
Slotkin, TA; Orband-Miller, L; Queen, KL
Published in: J Pharmacol Exp Ther
July 1987

The fetal and postnatal development of binding sites for [3H]nicotine was examined in brain regions of normal rats and rats whose mothers received nicotine injections or infusions, starting before fetal implantation (gestational day 4) and continuing to gestational day 20. The normal ontogenetic pattern of binding indicated a small but detectable concentration of sites during late gestation, and a substantial increase after birth, primarily during the period in which the majority of cholinergic synapses is forming. The adult pattern of regional selectivity of binding capabilities, namely midbrain + brainstem greater than cerebral cortex much greater than cerebellum, was not present at birth, but rather developed over the ensuing 3 weeks postpartum. Fetal exposure to nicotine produced an elevation in binding detectable during the course of drug exposure (gestational day 18), a finding similar to nicotine's effects in mature brain. However, examination of the subsequent developmental pattern of [3H]nicotine binding indicated a generalized disruption of receptor acquisition, in that alterations persisted far beyond the period in which drug exposure was terminated. The greatest effect was seen in a region relatively poor in receptor sites (cerebellum), and a larger stimulation was obtained with injected vs. infused nicotine. Because the cerebellum is the primary target for disruption of cellular development by prenatal nicotine exposure, these results are consistent with a primary teratologic action of the drug rather than direct effects on development of [3H]nicotine receptors.

Duke Scholars

Published In

J Pharmacol Exp Ther

ISSN

0022-3565

Publication Date

July 1987

Volume

242

Issue

1

Start / End Page

232 / 237

Location

United States

Related Subject Headings

  • Receptors, Nicotinic
  • Rats
  • Pregnancy
  • Pharmacology & Pharmacy
  • Nicotine
  • Mesencephalon
  • Maternal-Fetal Exchange
  • Injections, Subcutaneous
  • Infusion Pumps
  • Gestational Age
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Slotkin, T. A., Orband-Miller, L., & Queen, K. L. (1987). Development of [3H]nicotine binding sites in brain regions of rats exposed to nicotine prenatally via maternal injections or infusions. J Pharmacol Exp Ther, 242(1), 232–237.
Slotkin, T. A., L. Orband-Miller, and K. L. Queen. “Development of [3H]nicotine binding sites in brain regions of rats exposed to nicotine prenatally via maternal injections or infusions.J Pharmacol Exp Ther 242, no. 1 (July 1987): 232–37.
Slotkin, T. A., et al. “Development of [3H]nicotine binding sites in brain regions of rats exposed to nicotine prenatally via maternal injections or infusions.J Pharmacol Exp Ther, vol. 242, no. 1, July 1987, pp. 232–37.

Published In

J Pharmacol Exp Ther

ISSN

0022-3565

Publication Date

July 1987

Volume

242

Issue

1

Start / End Page

232 / 237

Location

United States

Related Subject Headings

  • Receptors, Nicotinic
  • Rats
  • Pregnancy
  • Pharmacology & Pharmacy
  • Nicotine
  • Mesencephalon
  • Maternal-Fetal Exchange
  • Injections, Subcutaneous
  • Infusion Pumps
  • Gestational Age