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Ordered phosphorylation of p42mapk by MAP kinase kinase.

Publication ,  Journal Article
Haystead, TA; Dent, P; Wu, J; Haystead, CM; Sturgill, TW
Published in: FEBS Lett
July 13, 1992

Preparation of milligram amounts of [32P]p42mapk, phosphorylated at Tyr185 or diphosphorylated at Tyr185/Thr183, for use as specific protein phosphatase substrates is described. Tyr- but not Thr-phosphorylated p42mapk, accumulates when ATP is limiting. Furthermore, Tyr185-phosphorylated p42mapk exhibits an apparent 10-fold decrease in apparent Km (46.6 +/- 6.6 nM) for MAP kinase kinase compared to that for the dephospho form (approximately 476 nM). We conclude that Tyr185 precedes Thr183 phosphorylation, and that this is prerequisite, dramatically increasing the affinity of p42mapk for MAP kinase kinase.

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Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

July 13, 1992

Volume

306

Issue

1

Start / End Page

17 / 22

Location

England

Related Subject Headings

  • Tyrosine
  • Threonine
  • Substrate Specificity
  • Recombinant Proteins
  • Rabbits
  • Protein-Tyrosine Kinases
  • Protein Serine-Threonine Kinases
  • Protein Kinases
  • Phosphorylation
  • Muscles
 

Citation

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Haystead, T. A., Dent, P., Wu, J., Haystead, C. M., & Sturgill, T. W. (1992). Ordered phosphorylation of p42mapk by MAP kinase kinase. FEBS Lett, 306(1), 17–22. https://doi.org/10.1016/0014-5793(92)80828-5
Haystead, T. A., P. Dent, J. Wu, C. M. Haystead, and T. W. Sturgill. “Ordered phosphorylation of p42mapk by MAP kinase kinase.FEBS Lett 306, no. 1 (July 13, 1992): 17–22. https://doi.org/10.1016/0014-5793(92)80828-5.
Haystead TA, Dent P, Wu J, Haystead CM, Sturgill TW. Ordered phosphorylation of p42mapk by MAP kinase kinase. FEBS Lett. 1992 Jul 13;306(1):17–22.
Haystead, T. A., et al. “Ordered phosphorylation of p42mapk by MAP kinase kinase.FEBS Lett, vol. 306, no. 1, July 1992, pp. 17–22. Pubmed, doi:10.1016/0014-5793(92)80828-5.
Haystead TA, Dent P, Wu J, Haystead CM, Sturgill TW. Ordered phosphorylation of p42mapk by MAP kinase kinase. FEBS Lett. 1992 Jul 13;306(1):17–22.
Journal cover image

Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

July 13, 1992

Volume

306

Issue

1

Start / End Page

17 / 22

Location

England

Related Subject Headings

  • Tyrosine
  • Threonine
  • Substrate Specificity
  • Recombinant Proteins
  • Rabbits
  • Protein-Tyrosine Kinases
  • Protein Serine-Threonine Kinases
  • Protein Kinases
  • Phosphorylation
  • Muscles