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Abi-2, a novel SH3-containing protein interacts with the c-Abl tyrosine kinase and modulates c-Abl transforming activity.

Publication ,  Journal Article
Dai, Z; Pendergast, AM
Published in: Genes Dev
November 1, 1995

A protein has been identified that interacts specifically with both the Src homologous 3 (SH3) domain and carboxy-terminal sequences of the c-Abl tyrosine kinase. The cDNA encoding the Abl interactor protein (Abi-2), was isolated from a human lymphocyte library using the yeast two-hybrid system with the Abl SH3 domain as bait. Abi-2 binds to c-Abl in vitro and in vivo. Abi-2 is a novel protein that contains an SH3 domain and proline-rich sequences critical for binding to c-Abl. A basic region in the amino terminus of Abi-2 is homologous to the DNA-binding sequence of homeo-domain proteins. We show that Abi-2 is a substrate for the c-Abl tyrosine kinase. Expression of an Abi-2 mutant protein that lacks sequences required for binding to the Abl SH3 domain but retains binding to the Abl carboxyl terminus activates the transforming capacity of c-Abl. The properties of Abi-2 are consistent with a dual role as regulator and potential effector of the c-Abl protein and suggest that Abi-2 may function as a tumor suppressor in mammalian cells.

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Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

November 1, 1995

Volume

9

Issue

21

Start / End Page

2569 / 2582

Location

United States

Related Subject Headings

  • src Homology Domains
  • Tissue Distribution
  • Substrate Specificity
  • Sequence Homology, Amino Acid
  • RNA
  • Proto-Oncogene Proteins c-abl
  • Protein-Tyrosine Kinases
  • Proline
  • Mutation
  • Molecular Sequence Data
 

Citation

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Dai, Z., & Pendergast, A. M. (1995). Abi-2, a novel SH3-containing protein interacts with the c-Abl tyrosine kinase and modulates c-Abl transforming activity. Genes Dev, 9(21), 2569–2582. https://doi.org/10.1101/gad.9.21.2569
Dai, Z., and A. M. Pendergast. “Abi-2, a novel SH3-containing protein interacts with the c-Abl tyrosine kinase and modulates c-Abl transforming activity.Genes Dev 9, no. 21 (November 1, 1995): 2569–82. https://doi.org/10.1101/gad.9.21.2569.
Dai, Z., and A. M. Pendergast. “Abi-2, a novel SH3-containing protein interacts with the c-Abl tyrosine kinase and modulates c-Abl transforming activity.Genes Dev, vol. 9, no. 21, Nov. 1995, pp. 2569–82. Pubmed, doi:10.1101/gad.9.21.2569.

Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

November 1, 1995

Volume

9

Issue

21

Start / End Page

2569 / 2582

Location

United States

Related Subject Headings

  • src Homology Domains
  • Tissue Distribution
  • Substrate Specificity
  • Sequence Homology, Amino Acid
  • RNA
  • Proto-Oncogene Proteins c-abl
  • Protein-Tyrosine Kinases
  • Proline
  • Mutation
  • Molecular Sequence Data