Skip to main content

Toxicity of methoxyacetic acid in cultured human luteal cells.

Publication ,  Journal Article
Almekinder, JL; Lennard, DE; Walmer, DK; Davis, BJ
Published in: Fundamental and applied toxicology : official journal of the Society of Toxicology
August 1997

Ethylene glycol monomethyl ether (EGME) and its proximate metabolite, 2-methoxyacetic acid (MAA), increase ovarian luteal cell progesterone production in the female rat in vivo and in cultured rat luteal cells in vitro, respectively. In order to better assess the potential hazard of EGME and MAA to women, these studies were conducted to determine whether the same concentrations of MAA increase progesterone in human luteinized granulosa cells as in rat luteal cells. Human cells were collected from healthy anonymous oocyte donors, washed, plated 25,000 viable cells per well, and treated with 10 IU hCG and 0-5 mM MAA for 6-48 hr. Progesterone in media was significantly elevated after 24 hr incubation at >/=1 mM MAA. MAA had no effect on ATP levels at 6 or 24 hr. Thus, MAA increased progesterone production in cultured human luteal cells at the same concentration as MAA increased progesterone in rat luteal cells. The implication is that EGME has the potential to alter ovarian luteal function in women. These data should be useful for determining the real health hazards and potential risks of EGME exposure.

Duke Scholars

Published In

Fundamental and applied toxicology : official journal of the Society of Toxicology

DOI

ISSN

0272-0590

Publication Date

August 1997

Volume

38

Issue

2

Start / End Page

191 / 194

Related Subject Headings

  • Toxicology
  • Solvents
  • Rats
  • Radioimmunoassay
  • Progesterone
  • Ovary
  • Hydrogen-Ion Concentration
  • Humans
  • Granulosa Cells
  • Female
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Almekinder, J. L., Lennard, D. E., Walmer, D. K., & Davis, B. J. (1997). Toxicity of methoxyacetic acid in cultured human luteal cells. Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology, 38(2), 191–194. https://doi.org/10.1006/faat.1997.2332
Almekinder, J. L., D. E. Lennard, D. K. Walmer, and B. J. Davis. “Toxicity of methoxyacetic acid in cultured human luteal cells.Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology 38, no. 2 (August 1997): 191–94. https://doi.org/10.1006/faat.1997.2332.
Almekinder JL, Lennard DE, Walmer DK, Davis BJ. Toxicity of methoxyacetic acid in cultured human luteal cells. Fundamental and applied toxicology : official journal of the Society of Toxicology. 1997 Aug;38(2):191–4.
Almekinder, J. L., et al. “Toxicity of methoxyacetic acid in cultured human luteal cells.Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology, vol. 38, no. 2, Aug. 1997, pp. 191–94. Epmc, doi:10.1006/faat.1997.2332.
Almekinder JL, Lennard DE, Walmer DK, Davis BJ. Toxicity of methoxyacetic acid in cultured human luteal cells. Fundamental and applied toxicology : official journal of the Society of Toxicology. 1997 Aug;38(2):191–194.

Published In

Fundamental and applied toxicology : official journal of the Society of Toxicology

DOI

ISSN

0272-0590

Publication Date

August 1997

Volume

38

Issue

2

Start / End Page

191 / 194

Related Subject Headings

  • Toxicology
  • Solvents
  • Rats
  • Radioimmunoassay
  • Progesterone
  • Ovary
  • Hydrogen-Ion Concentration
  • Humans
  • Granulosa Cells
  • Female