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Improved flow cytometric analysis of the budding yeast cell cycle.

Publication ,  Journal Article
Haase, SB; Reed, SI
Published in: Cell cycle (Georgetown, Tex.)
March 2002

The budding yeast, Saccharomyces cerevisiae has been a remarkably useful model system for the study of eukaryotic cell cycle regulation. Flow cytometric analysis of DNA content in budding yeast has become a standard tool for the analysis of cell cycle progression. However, popular protocols utilizing the DNA binding dye, propidium iodide, suffer from a number of drawbacks that confound accurate analysis by flow cytometry. Here we show the utility of the DNA binding dye, SYTOX Green, in the cell cycle analysis of yeast. Samples analyzed using SYTOX Green exhibited better coefficients of variation, improved linearity between DNA content and fluorescence, and decreased peak drift associated with changes in dye concentration, growth conditions or cell size.

Duke Scholars

Steven B. Haase
Author Steven B. Haase Biology

Published In

Cell cycle (Georgetown, Tex.)

EISSN

1551-4005

ISSN

1538-4101

Publication Date

March 2002

Volume

1

Issue

2

Start / End Page

132 / 136

Related Subject Headings

  • Saccharomyces cerevisiae
  • Propidium
  • Organic Chemicals
  • Fluorescent Dyes
  • Flow Cytometry
  • Developmental Biology
  • Coloring Agents
  • Cell Division
  • Cell Cycle
  • 3101 Biochemistry and cell biology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Haase, S. B., & Reed, S. I. (2002). Improved flow cytometric analysis of the budding yeast cell cycle. Cell Cycle (Georgetown, Tex.), 1(2), 132–136.
Haase, Steven B., and Steven I. Reed. “Improved flow cytometric analysis of the budding yeast cell cycle.” Cell Cycle (Georgetown, Tex.) 1, no. 2 (March 2002): 132–36.
Haase SB, Reed SI. Improved flow cytometric analysis of the budding yeast cell cycle. Cell cycle (Georgetown, Tex). 2002 Mar;1(2):132–6.
Haase, Steven B., and Steven I. Reed. “Improved flow cytometric analysis of the budding yeast cell cycle.” Cell Cycle (Georgetown, Tex.), vol. 1, no. 2, Mar. 2002, pp. 132–36.
Haase SB, Reed SI. Improved flow cytometric analysis of the budding yeast cell cycle. Cell cycle (Georgetown, Tex). 2002 Mar;1(2):132–136.

Published In

Cell cycle (Georgetown, Tex.)

EISSN

1551-4005

ISSN

1538-4101

Publication Date

March 2002

Volume

1

Issue

2

Start / End Page

132 / 136

Related Subject Headings

  • Saccharomyces cerevisiae
  • Propidium
  • Organic Chemicals
  • Fluorescent Dyes
  • Flow Cytometry
  • Developmental Biology
  • Coloring Agents
  • Cell Division
  • Cell Cycle
  • 3101 Biochemistry and cell biology