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Cytochalasins induce actin polymerization in human leukocytes.

Publication ,  Journal Article
Rao, KM; Padmanabhan, J; Cohen, HJ
Published in: Cell Motil Cytoskeleton
1992

We studied the effect of cytochalasins (B, D, and E) on the F-actin content in human neutrophils and lymphocytes using NBD-phallacidin labeling followed by flow cytometry. All three cytochalasins induced a concentration- and time-dependent increase in the F-actin content in both cell types. The order of potency was cytochalasin D greater than E greater than B. The increase in F-actin content was accompanied by a decrease in the G-actin content as measured by DNase I inhibition assay. These observations suggest that in intact cells cytochalasins may function differently compared to purified and semipurified systems, and their effects may be modified through other actin-binding or sequestering proteins. 2-deoxyglucose (20 mM) caused a decrease in the basal F-actin content and significantly reduced the change induced by the cytochalasins. These results suggest that the state of actin in intact cells is regulated by cytosolic ATP levels, primarily by the integrity of the glycolytic pathway. Based on these observations, we conclude that the mechanism of action of cytochalasins in intact cells is more complex than current models suggest.

Duke Scholars

Published In

Cell Motil Cytoskeleton

DOI

ISSN

0886-1544

Publication Date

1992

Volume

21

Issue

1

Start / End Page

58 / 64

Location

United States

Related Subject Headings

  • Polymers
  • Neutrophils
  • Lymphocytes
  • Humans
  • Flow Cytometry
  • Developmental Biology
  • Cytochalasins
  • Antimetabolites
  • Amanitins
  • Actins
 

Citation

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MLA
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Rao, K. M., Padmanabhan, J., & Cohen, H. J. (1992). Cytochalasins induce actin polymerization in human leukocytes. Cell Motil Cytoskeleton, 21(1), 58–64. https://doi.org/10.1002/cm.970210107
Rao, K. M., J. Padmanabhan, and H. J. Cohen. “Cytochalasins induce actin polymerization in human leukocytes.Cell Motil Cytoskeleton 21, no. 1 (1992): 58–64. https://doi.org/10.1002/cm.970210107.
Rao KM, Padmanabhan J, Cohen HJ. Cytochalasins induce actin polymerization in human leukocytes. Cell Motil Cytoskeleton. 1992;21(1):58–64.
Rao, K. M., et al. “Cytochalasins induce actin polymerization in human leukocytes.Cell Motil Cytoskeleton, vol. 21, no. 1, 1992, pp. 58–64. Pubmed, doi:10.1002/cm.970210107.
Rao KM, Padmanabhan J, Cohen HJ. Cytochalasins induce actin polymerization in human leukocytes. Cell Motil Cytoskeleton. 1992;21(1):58–64.
Journal cover image

Published In

Cell Motil Cytoskeleton

DOI

ISSN

0886-1544

Publication Date

1992

Volume

21

Issue

1

Start / End Page

58 / 64

Location

United States

Related Subject Headings

  • Polymers
  • Neutrophils
  • Lymphocytes
  • Humans
  • Flow Cytometry
  • Developmental Biology
  • Cytochalasins
  • Antimetabolites
  • Amanitins
  • Actins