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Enhancement of macrophage-mediated tumor cell killing by bacterial outer membrane proteins (porins).

Publication ,  Journal Article
Weinberg, JB; Ribi, E; Wheat, RW
Published in: Infect Immun
October 1983

Various microbial products are known to influence the function of mouse peritoneal macrophages. Lipopolysaccharide (LPS) and certain lipid A-associated proteins are known to enhance the tumoricidal effects of macrophages. The purpose of this study was to determine whether porins (outer membrane proteins) of Salmonella typhimurium G30/C21 would influence the activity of macrophages from lipid A-responsive and -unresponsive mice. Porins, extracted by a combined sodium dodecyl sulfate-EDTA method from cell walls, were free of LPS as determined by Limulus amebocyte lysate assay and appeared as a band at approximately 36,000 molecular weight on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In tumor cell killing assays done under LPS-free conditions, the porins in doses of 1 to 10 ng/ml enhanced the tumoricidal effect of macrophages from bacillus Calmette-Guérin-infected C3H/HeN or C3H/HeJ mice. Protein-free LPS enhanced the tumoricidal activity of macrophages from bacillus Calmette-Guérin-infected C3H/HeN but not C3H/HeJ mice. The tumoricidal-enhancing activity of protein-free LPS was blocked by the lipid A-binding antibiotic polymyxin B sulfate, but the effects of porins were not altered by the polymyxin B sulfate. These results suggest that porins, proteins known to alter membrane function, may alter macrophage function by interaction with macrophage membranes.

Duke Scholars

Published In

Infect Immun

DOI

ISSN

0019-9567

Publication Date

October 1983

Volume

42

Issue

1

Start / End Page

219 / 223

Location

United States

Related Subject Headings

  • Sarcoma, Experimental
  • Salmonella typhimurium
  • Porins
  • Polymyxin B
  • Microbiology
  • Mice, Inbred C3H
  • Mice
  • Membrane Proteins
  • Macrophages
  • Lipopolysaccharides
 

Citation

APA
Chicago
ICMJE
MLA
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Weinberg, J. B., Ribi, E., & Wheat, R. W. (1983). Enhancement of macrophage-mediated tumor cell killing by bacterial outer membrane proteins (porins). Infect Immun, 42(1), 219–223. https://doi.org/10.1128/iai.42.1.219-223.1983
Weinberg, J. B., E. Ribi, and R. W. Wheat. “Enhancement of macrophage-mediated tumor cell killing by bacterial outer membrane proteins (porins).Infect Immun 42, no. 1 (October 1983): 219–23. https://doi.org/10.1128/iai.42.1.219-223.1983.
Weinberg JB, Ribi E, Wheat RW. Enhancement of macrophage-mediated tumor cell killing by bacterial outer membrane proteins (porins). Infect Immun. 1983 Oct;42(1):219–23.
Weinberg, J. B., et al. “Enhancement of macrophage-mediated tumor cell killing by bacterial outer membrane proteins (porins).Infect Immun, vol. 42, no. 1, Oct. 1983, pp. 219–23. Pubmed, doi:10.1128/iai.42.1.219-223.1983.
Weinberg JB, Ribi E, Wheat RW. Enhancement of macrophage-mediated tumor cell killing by bacterial outer membrane proteins (porins). Infect Immun. 1983 Oct;42(1):219–223.

Published In

Infect Immun

DOI

ISSN

0019-9567

Publication Date

October 1983

Volume

42

Issue

1

Start / End Page

219 / 223

Location

United States

Related Subject Headings

  • Sarcoma, Experimental
  • Salmonella typhimurium
  • Porins
  • Polymyxin B
  • Microbiology
  • Mice, Inbred C3H
  • Mice
  • Membrane Proteins
  • Macrophages
  • Lipopolysaccharides