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Interleukin-1 and tumor necrosis factor alpha inhibit repair of the porcine meniscus in vitro.

Publication ,  Journal Article
Hennerbichler, A; Moutos, FT; Hennerbichler, D; Weinberg, JB; Guilak, F
Published in: Osteoarthritis Cartilage
September 2007

OBJECTIVE: Injury or removal of the knee meniscus leads to progressive joint degeneration, and current surgical therapies for meniscal tears seek to maximally preserve meniscal structure and function. However, the factors that influence intrinsic repair of the meniscus are not well understood. The goal of this study was to investigate the capacity of meniscus tissue to repair a simulated defect in vitro and to examine the effect of pro-inflammatory cytokines on this process. METHODS: Cylindrical explants were harvested from the outer one-third of medial porcine menisci. To simulate a full-thickness defect, a central core was removed and reinserted immediately into the defect. Explants were cultured for 2, 4, or 6 weeks in serum-containing media in the presence or absence of interleukin-1 (IL-1) or tumor necrosis factor alpha (TNF-alpha), and meniscal repair was investigated using mechanical testing and fluorescence confocal microscopy. RESULTS: Meniscal lesions in untreated samples showed a significant capacity for intrinsic repair in vitro, with increasing cell accumulation and repair strength over time in culture. In the presence of IL-1 or TNF-alpha, no repair was observed despite the presence of abundant viable cells. CONCLUSIONS: This study demonstrates that the meniscus exhibits an intrinsic repair response in vitro. However, the presence of pro-inflammatory cytokines completely inhibited repair. These findings suggest that increased levels of pro-inflammatory cytokines post-injury or under arthritic conditions may inhibit meniscal repair. Therefore, inhibition of these cytokines may provide a means of accelerating repair of damaged or injured menisci in vivo.

Duke Scholars

Published In

Osteoarthritis Cartilage

DOI

ISSN

1063-4584

Publication Date

September 2007

Volume

15

Issue

9

Start / End Page

1053 / 1060

Location

England

Related Subject Headings

  • Wound Healing
  • Tumor Necrosis Factor-alpha
  • Tibial Meniscus Injuries
  • Swine
  • Stress, Mechanical
  • Microscopy, Confocal
  • Menisci, Tibial
  • Interleukin-1
  • In Vitro Techniques
  • Fluorescence
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hennerbichler, A., Moutos, F. T., Hennerbichler, D., Weinberg, J. B., & Guilak, F. (2007). Interleukin-1 and tumor necrosis factor alpha inhibit repair of the porcine meniscus in vitro. Osteoarthritis Cartilage, 15(9), 1053–1060. https://doi.org/10.1016/j.joca.2007.03.003
Hennerbichler, A., F. T. Moutos, D. Hennerbichler, J. B. Weinberg, and F. Guilak. “Interleukin-1 and tumor necrosis factor alpha inhibit repair of the porcine meniscus in vitro.Osteoarthritis Cartilage 15, no. 9 (September 2007): 1053–60. https://doi.org/10.1016/j.joca.2007.03.003.
Hennerbichler A, Moutos FT, Hennerbichler D, Weinberg JB, Guilak F. Interleukin-1 and tumor necrosis factor alpha inhibit repair of the porcine meniscus in vitro. Osteoarthritis Cartilage. 2007 Sep;15(9):1053–60.
Hennerbichler, A., et al. “Interleukin-1 and tumor necrosis factor alpha inhibit repair of the porcine meniscus in vitro.Osteoarthritis Cartilage, vol. 15, no. 9, Sept. 2007, pp. 1053–60. Pubmed, doi:10.1016/j.joca.2007.03.003.
Hennerbichler A, Moutos FT, Hennerbichler D, Weinberg JB, Guilak F. Interleukin-1 and tumor necrosis factor alpha inhibit repair of the porcine meniscus in vitro. Osteoarthritis Cartilage. 2007 Sep;15(9):1053–1060.
Journal cover image

Published In

Osteoarthritis Cartilage

DOI

ISSN

1063-4584

Publication Date

September 2007

Volume

15

Issue

9

Start / End Page

1053 / 1060

Location

England

Related Subject Headings

  • Wound Healing
  • Tumor Necrosis Factor-alpha
  • Tibial Meniscus Injuries
  • Swine
  • Stress, Mechanical
  • Microscopy, Confocal
  • Menisci, Tibial
  • Interleukin-1
  • In Vitro Techniques
  • Fluorescence