Skip to main content

Effect of a fullerene water suspension on bacterial phospholipids and membrane phase behavior.

Publication ,  Journal Article
Fang, J; Lyon, DY; Wiesner, MR; Dong, J; Alvarez, PJJ
Published in: Environmental science & technology
April 2007

Several fullerene-based nanomaterials generate reactive oxygen species that can damage cells. In this study, we investigated the effect of buckminsterfullerene (C60) introduced as colloidal aggregates in water (nC60) on bacterial membrane lipid composition and phase behavior. Pseudomonas putida (Gram-negative) and Bacillus subtilis (Gram-positive) responded to nC60 by altering membrane lipid composition, phase transition temperature, and membrane fluidity. P. putida decreased its levels of unsaturated fatty acids and increased the proportions of cyclopropane fatty acids in the presence of nC60, possibly to protect the bacterial membrane from oxidative stress. Fourier transform infrared spectroscopy measurement of intact bacterial cells showed slightly increased phase transition temperatures (Tm) and increased membrane fluidity for cells grown in the presence of high, growth-inhibiting concentrations (0.5 mg L(-1)) of nC60. B. subtilis responded to a low dose of nC6o (0.01 mg L(-1)) by significantly increasing the levels of iso- and anteiso-branched fatty acids (from 5.8 to 31.5% and 12.9 to 32.3% of total fatty acids, respectively) and to a high, growth-inhibiting concentration of nC60 (0.75 mg L-1) by increasing synthesis of monounsaturated fatty acids. In contrast to P. putida, B. subtilis response was a decrease in Tm and an increase in membrane fluidity. These findings represent the first demonstrated physiological adaptation response of bacteria to a manufactured nanomaterial, and they showthat response inlipid composition and membrane phase behavior depends on both the nC60 concentration and the cell wall morphology.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Environmental science & technology

DOI

EISSN

1520-5851

ISSN

0013-936X

Publication Date

April 2007

Volume

41

Issue

7

Start / End Page

2636 / 2642

Related Subject Headings

  • Temperature
  • Spectroscopy, Fourier Transform Infrared
  • Pseudomonas putida
  • Membrane Lipids
  • Fullerenes
  • Environmental Sciences
  • Dose-Response Relationship, Drug
  • Colloids
  • Cell Membrane
  • Bacillus subtilis
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Fang, J., Lyon, D. Y., Wiesner, M. R., Dong, J., & Alvarez, P. J. J. (2007). Effect of a fullerene water suspension on bacterial phospholipids and membrane phase behavior. Environmental Science & Technology, 41(7), 2636–2642. https://doi.org/10.1021/es062181w
Fang, Jiasong, Delina Y. Lyon, Mark R. Wiesner, Jinping Dong, and Pedro J. J. Alvarez. “Effect of a fullerene water suspension on bacterial phospholipids and membrane phase behavior.Environmental Science & Technology 41, no. 7 (April 2007): 2636–42. https://doi.org/10.1021/es062181w.
Fang J, Lyon DY, Wiesner MR, Dong J, Alvarez PJJ. Effect of a fullerene water suspension on bacterial phospholipids and membrane phase behavior. Environmental science & technology. 2007 Apr;41(7):2636–42.
Fang, Jiasong, et al. “Effect of a fullerene water suspension on bacterial phospholipids and membrane phase behavior.Environmental Science & Technology, vol. 41, no. 7, Apr. 2007, pp. 2636–42. Epmc, doi:10.1021/es062181w.
Fang J, Lyon DY, Wiesner MR, Dong J, Alvarez PJJ. Effect of a fullerene water suspension on bacterial phospholipids and membrane phase behavior. Environmental science & technology. 2007 Apr;41(7):2636–2642.

Published In

Environmental science & technology

DOI

EISSN

1520-5851

ISSN

0013-936X

Publication Date

April 2007

Volume

41

Issue

7

Start / End Page

2636 / 2642

Related Subject Headings

  • Temperature
  • Spectroscopy, Fourier Transform Infrared
  • Pseudomonas putida
  • Membrane Lipids
  • Fullerenes
  • Environmental Sciences
  • Dose-Response Relationship, Drug
  • Colloids
  • Cell Membrane
  • Bacillus subtilis