Degradation of proteoglycan in articular cartilage.
Adult rabbit articular cartilage was labelled in vivo over 48 h with [35S]sulphate and was then incubated in organ culture at pH 7.2. Approx. 65% of the tissue content of [35S]proteoglycan was released into the culture medium during the first 48 h of incubation. The average molecular size of the released proteoglycans, as assessed by fractionation on Sepharose 2B/CL and 4B/Cl, was only slightly smaller than that of the proteoglycans extracted from non-cultured cartilage with 4 M guanidine HCl. The percentage of released proteoglycans and extracted proteoglycans which formed aggregates with hyaluronic acid was approx. 25% and 75%, respectively. The results indicate that proteoglycan degradation in adult articular cartilage is initiated by a limited proteolysis of subunit core protein, with the production of non-aggregating species which diffuse readily from the tissue.
Duke Scholars
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- Rabbits
- Proteoglycans
- Peptide Hydrolases
- Organ Culture Techniques
- Molecular Weight
- Hydrolysis
- Hydrogen-Ion Concentration
- Hyaluronic Acid
- Cartilage, Articular
- Animals
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Rabbits
- Proteoglycans
- Peptide Hydrolases
- Organ Culture Techniques
- Molecular Weight
- Hydrolysis
- Hydrogen-Ion Concentration
- Hyaluronic Acid
- Cartilage, Articular
- Animals