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Amino acid uptake by isolated renal brush border membrane vesicles in various buffers.

Publication ,  Journal Article
Foreman, JW; Wald, H; Reynolds, RA; Segal, S
Published in: Biochim Biophys Acta
August 6, 1981

The uptake of amino acids by isolated rat renal brush border membrane vesicles in a modified Krebs-Ringer bicarbonate buffer and a phosphate buffer was compared to the uptake in the standard membrane vesicle buffer, Tris-Hepes-mannitol. The uptake in the modified Krebs-Ringer bicarbonate buffer was similar to that in the Tris-Hepes-mannitol buffer. Removal of the ionic constituents other than NaCl and NaHCO3 in the modified Krebs-Ringer bicarbonate buffer (KCl, CaCl2, KH2PO4 and MgSO4) did not affect the amino acid uptake by the isolated membrane vesicles. The timed uptake of proline under sodium gradient conditions in a phosphate buffer had a markedly dampened overshoot. Kinetic analysis of the initial rate of proline uptake in a phosphate buffer compared to a Tris-Herpes-mannitol buffer showed two entry systems for proline in each buffer with similar Km values, but the maximal rate of transport (V) for each system in the phosphate buffer was much lower than that in the Tris-Hepes-mannitol buffer. From these data, phosphate buffer does not appear to be a suitable medium for the study of amino acid uptake by isolated brush border membrane vesicles.

Duke Scholars

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

August 6, 1981

Volume

646

Issue

1

Start / End Page

188 / 192

Location

Netherlands

Related Subject Headings

  • Tromethamine
  • Rats
  • Proline
  • Phosphates
  • Microvilli
  • Male
  • Lysine
  • Kinetics
  • Kidney
  • HEPES
 

Citation

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Foreman, J. W., Wald, H., Reynolds, R. A., & Segal, S. (1981). Amino acid uptake by isolated renal brush border membrane vesicles in various buffers. Biochim Biophys Acta, 646(1), 188–192. https://doi.org/10.1016/0005-2736(81)90287-x
Foreman, J. W., H. Wald, R. A. Reynolds, and S. Segal. “Amino acid uptake by isolated renal brush border membrane vesicles in various buffers.Biochim Biophys Acta 646, no. 1 (August 6, 1981): 188–92. https://doi.org/10.1016/0005-2736(81)90287-x.
Foreman JW, Wald H, Reynolds RA, Segal S. Amino acid uptake by isolated renal brush border membrane vesicles in various buffers. Biochim Biophys Acta. 1981 Aug 6;646(1):188–92.
Foreman, J. W., et al. “Amino acid uptake by isolated renal brush border membrane vesicles in various buffers.Biochim Biophys Acta, vol. 646, no. 1, Aug. 1981, pp. 188–92. Pubmed, doi:10.1016/0005-2736(81)90287-x.
Foreman JW, Wald H, Reynolds RA, Segal S. Amino acid uptake by isolated renal brush border membrane vesicles in various buffers. Biochim Biophys Acta. 1981 Aug 6;646(1):188–192.

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

August 6, 1981

Volume

646

Issue

1

Start / End Page

188 / 192

Location

Netherlands

Related Subject Headings

  • Tromethamine
  • Rats
  • Proline
  • Phosphates
  • Microvilli
  • Male
  • Lysine
  • Kinetics
  • Kidney
  • HEPES