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Insulin-like growth factor-I gene expression in the tibial epiphyseal growth plate of growth hormone-treated uremic rats.

Publication ,  Journal Article
Hanna, JD; Santos, F; Foreman, JW; Chan, JC; Han, VK
Published in: Kidney Int
May 1995

To identify the molecular mechanisms involved in long bone growth of uremic animals, we evaluated the effects of recombinant human growth hormone (rhGH) supplementation on whole body growth, growth plate morphometrics, and insulin-like growth factor-I (IGF-I) gene expression in the tibial epiphyseal growth plates of uremic rats. Uremia was induced by a two-stage subtotal nephrectomy (Nx) of 30-day-old rats, followed by rhGH (N = 6) or saline (N = 6) treatment from day 56 to day 70 of age. Controls (N = 4) were sham decapsulated. Treatment with rhGH on Nx animals caused: (1) a significant increase in weight, (2) longitudinal growth similar to controls, and (3) increased total growth plate width predominantly due to an increase in hypertrophic zone width. rhGH increased IGF-I mRNA abundance in both zones, but the increase was greater in the proliferative zone. These changes were accompanied by concomitant alterations in IGF-I immunoreactivity. In uremic animals, therefore, rhGH treatment induces local IGF-I gene expression in the growth plate and increases the hypertrophic zone width but not the proliferative zone width. The latter suggests resistance to IGF-I action in that zone.

Duke Scholars

Published In

Kidney Int

DOI

ISSN

0085-2538

Publication Date

May 1995

Volume

47

Issue

5

Start / End Page

1374 / 1382

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Uremia
  • Tibia
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Male
  • Insulin-Like Growth Factor I
  • In Situ Hybridization
  • Immunohistochemistry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hanna, J. D., Santos, F., Foreman, J. W., Chan, J. C., & Han, V. K. (1995). Insulin-like growth factor-I gene expression in the tibial epiphyseal growth plate of growth hormone-treated uremic rats. Kidney Int, 47(5), 1374–1382. https://doi.org/10.1038/ki.1995.193
Hanna, J. D., F. Santos, J. W. Foreman, J. C. Chan, and V. K. Han. “Insulin-like growth factor-I gene expression in the tibial epiphyseal growth plate of growth hormone-treated uremic rats.Kidney Int 47, no. 5 (May 1995): 1374–82. https://doi.org/10.1038/ki.1995.193.
Hanna JD, Santos F, Foreman JW, Chan JC, Han VK. Insulin-like growth factor-I gene expression in the tibial epiphyseal growth plate of growth hormone-treated uremic rats. Kidney Int. 1995 May;47(5):1374–82.
Hanna, J. D., et al. “Insulin-like growth factor-I gene expression in the tibial epiphyseal growth plate of growth hormone-treated uremic rats.Kidney Int, vol. 47, no. 5, May 1995, pp. 1374–82. Pubmed, doi:10.1038/ki.1995.193.
Hanna JD, Santos F, Foreman JW, Chan JC, Han VK. Insulin-like growth factor-I gene expression in the tibial epiphyseal growth plate of growth hormone-treated uremic rats. Kidney Int. 1995 May;47(5):1374–1382.
Journal cover image

Published In

Kidney Int

DOI

ISSN

0085-2538

Publication Date

May 1995

Volume

47

Issue

5

Start / End Page

1374 / 1382

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Uremia
  • Tibia
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Male
  • Insulin-Like Growth Factor I
  • In Situ Hybridization
  • Immunohistochemistry