Skip to main content
Journal cover image

Effect of acidosis on glutamine transport by isolated rat renal brush-border and basolateral-membrane vesicles.

Publication ,  Journal Article
Foreman, JW; Reynolds, RA; Ginkinger, K; Segal, S
Published in: Biochem J
June 15, 1983

Glutamine uptake was examined in isolated renal brush-border and basolateral-membrane vesicles from control and acidotic rats. In brush-border vesicles from acidotic animals, there was a significant increase in the initial rate of glutamine uptake compared with that in controls. Lowering the pH of the medium increased the initial rate of glutamine uptake in brush-border vesicles from acidotic, but not from control, rats. In brush-border vesicles from both groups of animals, two saturable transport systems mediated glutamine uptake. There was a 2-fold increase in the Vmax. of the low-affinity high-capacity system in the brush-border vesicles from the acidotic animals compared with that from control animals, with no alteration in the other kinetic parameters. There was no difference in glutamine uptake by the two saturable transport systems in basolateral vesicles from control and acidotic animals. Lowering the incubation-medium pH increased the uptake of glutamine by basolateral vesicles from both control and acidotic rats to a similar extent. The data indicate that during acidosis there are alterations in glutamine transport by both the basolateral and brush-border membrane which could enhance its uptake by the renal-tubule cell for use in ammoniagenesis.

Duke Scholars

Published In

Biochem J

DOI

ISSN

0264-6021

Publication Date

June 15, 1983

Volume

212

Issue

3

Start / End Page

713 / 720

Location

England

Related Subject Headings

  • Rats, Inbred Strains
  • Rats
  • Microvilli
  • Male
  • Kinetics
  • Kidney
  • Hydrogen-Ion Concentration
  • Glutamine
  • Cell Membrane
  • Biological Transport
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Foreman, J. W., Reynolds, R. A., Ginkinger, K., & Segal, S. (1983). Effect of acidosis on glutamine transport by isolated rat renal brush-border and basolateral-membrane vesicles. Biochem J, 212(3), 713–720. https://doi.org/10.1042/bj2120713
Foreman, J. W., R. A. Reynolds, K. Ginkinger, and S. Segal. “Effect of acidosis on glutamine transport by isolated rat renal brush-border and basolateral-membrane vesicles.Biochem J 212, no. 3 (June 15, 1983): 713–20. https://doi.org/10.1042/bj2120713.
Foreman JW, Reynolds RA, Ginkinger K, Segal S. Effect of acidosis on glutamine transport by isolated rat renal brush-border and basolateral-membrane vesicles. Biochem J. 1983 Jun 15;212(3):713–20.
Foreman, J. W., et al. “Effect of acidosis on glutamine transport by isolated rat renal brush-border and basolateral-membrane vesicles.Biochem J, vol. 212, no. 3, June 1983, pp. 713–20. Pubmed, doi:10.1042/bj2120713.
Foreman JW, Reynolds RA, Ginkinger K, Segal S. Effect of acidosis on glutamine transport by isolated rat renal brush-border and basolateral-membrane vesicles. Biochem J. 1983 Jun 15;212(3):713–720.
Journal cover image

Published In

Biochem J

DOI

ISSN

0264-6021

Publication Date

June 15, 1983

Volume

212

Issue

3

Start / End Page

713 / 720

Location

England

Related Subject Headings

  • Rats, Inbred Strains
  • Rats
  • Microvilli
  • Male
  • Kinetics
  • Kidney
  • Hydrogen-Ion Concentration
  • Glutamine
  • Cell Membrane
  • Biological Transport