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Reduced spontaneous relaxation in immature guinea pig airway smooth muscle is associated with increased prostanoid release.

Publication ,  Journal Article
Wang, L; Pozzato, V; Turato, G; Madamanchi, A; Murphy, TM; Chitano, P
Published in: Am J Physiol Lung Cell Mol Physiol
May 2008

Airway smooth muscle (ASM) from infant guinea pigs has less spontaneous relaxation during stimulation than ASM from adults. Inhibition of cyclooxygenase (COX), which catalyzes the production of prostanoids, increases this relaxation in infant ASM and abolishes age differences, thus suggesting that prostanoids reduce relaxation in infant ASM. In this study, we investigated whether leukotrienes are also involved in reducing spontaneous relaxation; whether the two COX isoforms, COX-1 and COX-2, differentially regulate spontaneous relaxation; and whether prostanoid release is developmentally regulated in guinea pig ASM. In different age groups, we measured relaxation during and after electrical stimulation in tracheal strips as well as prostanoid release from tracheal segments. Relaxation was studied in the absence and in the presence of a lipoxygenase inhibitor, a cysteinyl leukotriene receptor-1 antagonist, a COX-1 inhibitor, or a COX-2 inhibitor. We found that inhibition of lipoxygenase or cysteinyl leukotriene receptor-1 antagonism did not increase spontaneous relaxation at any age, thus excluding a role for leukotrienes in this phenomenon. Inhibition of COX-2, but not COX-1, promoted spontaneous relaxation. The basal release of prostanoids was more abundant in tissue from infant animals and decreased significantly with age. Thromboxane B2 was the most abundant metabolite released at all ages. Electrical stimulation and epithelium removal did not affect the age difference in prostanoid release. We conclude that increased basal prostanoid release contributes to the reduced spontaneous relaxation in immature guinea pig ASM compared with older animals. By regulating ASM relaxation, prostanoids may play a role in the airway hyperresponsiveness at a young age.

Duke Scholars

Published In

Am J Physiol Lung Cell Mol Physiol

DOI

ISSN

1040-0605

Publication Date

May 2008

Volume

294

Issue

5

Start / End Page

L964 / L973

Location

United States

Related Subject Headings

  • Trachea
  • Sulfones
  • Respiratory System
  • Pyrazoles
  • Prostaglandins
  • Muscle, Smooth
  • Leukotrienes
  • Isoxazoles
  • Guinea Pigs
  • Cyclooxygenase Inhibitors
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wang, L., Pozzato, V., Turato, G., Madamanchi, A., Murphy, T. M., & Chitano, P. (2008). Reduced spontaneous relaxation in immature guinea pig airway smooth muscle is associated with increased prostanoid release. Am J Physiol Lung Cell Mol Physiol, 294(5), L964–L973. https://doi.org/10.1152/ajplung.00401.2007
Wang, Lu, Valeria Pozzato, Graziella Turato, Aasakiran Madamanchi, Thomas M. Murphy, and Pasquale Chitano. “Reduced spontaneous relaxation in immature guinea pig airway smooth muscle is associated with increased prostanoid release.Am J Physiol Lung Cell Mol Physiol 294, no. 5 (May 2008): L964–73. https://doi.org/10.1152/ajplung.00401.2007.
Wang L, Pozzato V, Turato G, Madamanchi A, Murphy TM, Chitano P. Reduced spontaneous relaxation in immature guinea pig airway smooth muscle is associated with increased prostanoid release. Am J Physiol Lung Cell Mol Physiol. 2008 May;294(5):L964–73.
Wang, Lu, et al. “Reduced spontaneous relaxation in immature guinea pig airway smooth muscle is associated with increased prostanoid release.Am J Physiol Lung Cell Mol Physiol, vol. 294, no. 5, May 2008, pp. L964–73. Pubmed, doi:10.1152/ajplung.00401.2007.
Wang L, Pozzato V, Turato G, Madamanchi A, Murphy TM, Chitano P. Reduced spontaneous relaxation in immature guinea pig airway smooth muscle is associated with increased prostanoid release. Am J Physiol Lung Cell Mol Physiol. 2008 May;294(5):L964–L973.

Published In

Am J Physiol Lung Cell Mol Physiol

DOI

ISSN

1040-0605

Publication Date

May 2008

Volume

294

Issue

5

Start / End Page

L964 / L973

Location

United States

Related Subject Headings

  • Trachea
  • Sulfones
  • Respiratory System
  • Pyrazoles
  • Prostaglandins
  • Muscle, Smooth
  • Leukotrienes
  • Isoxazoles
  • Guinea Pigs
  • Cyclooxygenase Inhibitors