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Adjacent positioning of cellular structures enabled by a Cdc42 GTPase-activating protein-mediated zone of inhibition.

Publication ,  Journal Article
Tong, Z; Gao, X-D; Howell, AS; Bose, I; Lew, DJ; Bi, E
Published in: J Cell Biol
December 31, 2007

Cells of the budding yeast Saccharomyces cerevisiae are born carrying localized transmembrane landmark proteins that guide the subsequent establishment of a polarity axis and hence polarized growth to form a bud in the next cell cycle. In haploid cells, the relevant landmark proteins are concentrated at the site of the preceding cell division, to which they recruit Cdc24, the guanine nucleotide exchange factor for the conserved polarity regulator Cdc42. However, instead of polarizing at the division site, the new polarity axis is directed next to but not overlapping that site. Here, we show that the Cdc42 guanosine triphosphatase-activating protein (GAP) Rga1 establishes an exclusion zone at the division site that blocks subsequent polarization within that site. In the absence of localized Rga1 GAP activity, new buds do in fact form within the old division site. Thus, Cdc42 activators and GAPs establish concentric zones of action such that polarization is directed to occur adjacent to but not within the previous cell division site.

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Published In

J Cell Biol

DOI

EISSN

1540-8140

Publication Date

December 31, 2007

Volume

179

Issue

7

Start / End Page

1375 / 1384

Location

United States

Related Subject Headings

  • cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Gene Expression Regulation, Fungal
  • GTPase-Activating Proteins
  • Developmental Biology
  • Cell Polarity
  • Cell Division
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
 

Citation

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Tong, Z., Gao, X.-D., Howell, A. S., Bose, I., Lew, D. J., & Bi, E. (2007). Adjacent positioning of cellular structures enabled by a Cdc42 GTPase-activating protein-mediated zone of inhibition. J Cell Biol, 179(7), 1375–1384. https://doi.org/10.1083/jcb.200705160
Tong, Zongtian, Xiang-Dong Gao, Audrey S. Howell, Indrani Bose, Daniel J. Lew, and Erfei Bi. “Adjacent positioning of cellular structures enabled by a Cdc42 GTPase-activating protein-mediated zone of inhibition.J Cell Biol 179, no. 7 (December 31, 2007): 1375–84. https://doi.org/10.1083/jcb.200705160.
Tong Z, Gao X-D, Howell AS, Bose I, Lew DJ, Bi E. Adjacent positioning of cellular structures enabled by a Cdc42 GTPase-activating protein-mediated zone of inhibition. J Cell Biol. 2007 Dec 31;179(7):1375–84.
Tong, Zongtian, et al. “Adjacent positioning of cellular structures enabled by a Cdc42 GTPase-activating protein-mediated zone of inhibition.J Cell Biol, vol. 179, no. 7, Dec. 2007, pp. 1375–84. Pubmed, doi:10.1083/jcb.200705160.
Tong Z, Gao X-D, Howell AS, Bose I, Lew DJ, Bi E. Adjacent positioning of cellular structures enabled by a Cdc42 GTPase-activating protein-mediated zone of inhibition. J Cell Biol. 2007 Dec 31;179(7):1375–1384.

Published In

J Cell Biol

DOI

EISSN

1540-8140

Publication Date

December 31, 2007

Volume

179

Issue

7

Start / End Page

1375 / 1384

Location

United States

Related Subject Headings

  • cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Gene Expression Regulation, Fungal
  • GTPase-Activating Proteins
  • Developmental Biology
  • Cell Polarity
  • Cell Division
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences