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Structure of the F-spondin domain of mindin, an integrin ligand and pattern recognition molecule.

Publication ,  Journal Article
Li, Y; Cao, C; Jia, W; Yu, L; Mo, M; Wang, Q; Huang, Y; Lim, J-M; Ishihara, M; Wells, L; Azadi, P; Robinson, H; He, Y-W; Zhang, L; Mariuzza, RA
Published in: EMBO J
February 4, 2009

Mindin (spondin-2) is an extracellular matrix protein of unknown structure that is required for efficient T-cell priming by dendritic cells. Additionally, mindin functions as a pattern recognition molecule for initiating innate immune responses. These dual functions are mediated by interactions with integrins and microbial pathogens, respectively. Mindin comprises an N-terminal F-spondin (FS) domain and C-terminal thrombospondin type 1 repeat (TSR). We determined the structure of the FS domain at 1.8-A resolution. The structure revealed an eight-stranded antiparallel beta-sandwich motif resembling that of membrane-targeting C2 domains, including a bound calcium ion. We demonstrated that the FS domain mediates integrin binding and identified the binding site by mutagenesis. The mindin FS domain therefore represents a new integrin ligand. We further showed that mindin recognizes lipopolysaccharide (LPS) through its TSR domain, and obtained evidence that C-mannosylation of the TSR influences LPS binding. Through these dual interactions, the FS and TSR domains of mindin promote activation of both adaptive and innate immune responses.

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Published In

EMBO J

DOI

EISSN

1460-2075

Publication Date

February 4, 2009

Volume

28

Issue

3

Start / End Page

286 / 297

Location

England

Related Subject Headings

  • Static Electricity
  • Repetitive Sequences, Amino Acid
  • Receptors, Pattern Recognition
  • Protein Structure, Tertiary
  • Protein Structure, Secondary
  • Protein Binding
  • Molecular Sequence Data
  • Models, Molecular
  • Mice
  • Mass Spectrometry
 

Citation

APA
Chicago
ICMJE
MLA
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Li, Y., Cao, C., Jia, W., Yu, L., Mo, M., Wang, Q., … Mariuzza, R. A. (2009). Structure of the F-spondin domain of mindin, an integrin ligand and pattern recognition molecule. EMBO J, 28(3), 286–297. https://doi.org/10.1038/emboj.2008.288
Li, Yili, Chunzhang Cao, Wei Jia, Lily Yu, Min Mo, Qian Wang, Yuping Huang, et al. “Structure of the F-spondin domain of mindin, an integrin ligand and pattern recognition molecule.EMBO J 28, no. 3 (February 4, 2009): 286–97. https://doi.org/10.1038/emboj.2008.288.
Li Y, Cao C, Jia W, Yu L, Mo M, Wang Q, et al. Structure of the F-spondin domain of mindin, an integrin ligand and pattern recognition molecule. EMBO J. 2009 Feb 4;28(3):286–97.
Li, Yili, et al. “Structure of the F-spondin domain of mindin, an integrin ligand and pattern recognition molecule.EMBO J, vol. 28, no. 3, Feb. 2009, pp. 286–97. Pubmed, doi:10.1038/emboj.2008.288.
Li Y, Cao C, Jia W, Yu L, Mo M, Wang Q, Huang Y, Lim J-M, Ishihara M, Wells L, Azadi P, Robinson H, He Y-W, Zhang L, Mariuzza RA. Structure of the F-spondin domain of mindin, an integrin ligand and pattern recognition molecule. EMBO J. 2009 Feb 4;28(3):286–297.

Published In

EMBO J

DOI

EISSN

1460-2075

Publication Date

February 4, 2009

Volume

28

Issue

3

Start / End Page

286 / 297

Location

England

Related Subject Headings

  • Static Electricity
  • Repetitive Sequences, Amino Acid
  • Receptors, Pattern Recognition
  • Protein Structure, Tertiary
  • Protein Structure, Secondary
  • Protein Binding
  • Molecular Sequence Data
  • Models, Molecular
  • Mice
  • Mass Spectrometry