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Immunohistochemical localization of ciliary neurotrophic factor receptor alpha expression in the rat nervous system.

Publication ,  Journal Article
MacLennan, AJ; Vinson, EN; Marks, L; McLaurin, DL; Pfeifer, M; Lee, N
Published in: J Neurosci
January 15, 1996

Ciliary neurotrophic factor receptor alpha (CNTFR alpha) is essential for normal embryonic development and may be involved in postnatal and adult neuronal maintenance. In addition, a rapidly growing body of evidence suggests that CNTFR alpha serves as a site of action for future growth factor therapeutics capable of treating a wide variety of disorders resulting from neuronal loss. We raised two polyclonal, anti-CNTFR alpha antisera against synthetic peptides corresponding to independent regions of rat CNTFR alpha. Western blot and immunohistochemical analyses indicated that affinity-purified preparations of both antisera specifically recognize CNTFR alpha. In the adult brain, the highest levels of CNTFR alpha immunoreactivity were found in the perikarya, dendrites and, occasionally, the axons of several distinct classes of neurons including hippocampal formation neurons, some sensory neurons, and many neurons involved in motor control. CNTFR alpha immunoreactivity also was concentrated in the following: perikarya, dendrites, and axons of ventral horn motor neurons in adult spinal cord; perikarya and axons of adult dorsal root ganglion neurons; and axons in adult peripheral nerve. In embryonic tissue, the highest levels of CNTFR alpha immunoreactivity were observed in differentiating neurons and their processes. Therefore, the present data suggest that CNTFR alpha serves several diverse functions in adulthood and during development.

Duke Scholars

Published In

J Neurosci

DOI

ISSN

0270-6474

Publication Date

January 15, 1996

Volume

16

Issue

2

Start / End Page

621 / 630

Location

United States

Related Subject Headings

  • Spinal Cord
  • Receptors, Nerve Growth Factor
  • Receptor, Ciliary Neurotrophic Factor
  • Rats
  • Peripheral Nerves
  • Neurology & Neurosurgery
  • Nerve Tissue Proteins
  • Muscles
  • Molecular Sequence Data
  • Immunohistochemistry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
MacLennan, A. J., Vinson, E. N., Marks, L., McLaurin, D. L., Pfeifer, M., & Lee, N. (1996). Immunohistochemical localization of ciliary neurotrophic factor receptor alpha expression in the rat nervous system. J Neurosci, 16(2), 621–630. https://doi.org/10.1523/JNEUROSCI.16-02-00621.1996
MacLennan, A. J., E. N. Vinson, L. Marks, D. L. McLaurin, M. Pfeifer, and N. Lee. “Immunohistochemical localization of ciliary neurotrophic factor receptor alpha expression in the rat nervous system.J Neurosci 16, no. 2 (January 15, 1996): 621–30. https://doi.org/10.1523/JNEUROSCI.16-02-00621.1996.
MacLennan AJ, Vinson EN, Marks L, McLaurin DL, Pfeifer M, Lee N. Immunohistochemical localization of ciliary neurotrophic factor receptor alpha expression in the rat nervous system. J Neurosci. 1996 Jan 15;16(2):621–30.
MacLennan, A. J., et al. “Immunohistochemical localization of ciliary neurotrophic factor receptor alpha expression in the rat nervous system.J Neurosci, vol. 16, no. 2, Jan. 1996, pp. 621–30. Pubmed, doi:10.1523/JNEUROSCI.16-02-00621.1996.
MacLennan AJ, Vinson EN, Marks L, McLaurin DL, Pfeifer M, Lee N. Immunohistochemical localization of ciliary neurotrophic factor receptor alpha expression in the rat nervous system. J Neurosci. 1996 Jan 15;16(2):621–630.

Published In

J Neurosci

DOI

ISSN

0270-6474

Publication Date

January 15, 1996

Volume

16

Issue

2

Start / End Page

621 / 630

Location

United States

Related Subject Headings

  • Spinal Cord
  • Receptors, Nerve Growth Factor
  • Receptor, Ciliary Neurotrophic Factor
  • Rats
  • Peripheral Nerves
  • Neurology & Neurosurgery
  • Nerve Tissue Proteins
  • Muscles
  • Molecular Sequence Data
  • Immunohistochemistry