Skip to main content

Photodynamic therapy of Barrett's esophagus: ablation of Barrett's mucosa and reduction in p53 protein expression after treatment.

Publication ,  Journal Article
Panjehpour, M; Coppola, D; Overholt, BF; Vo-Dinh, T; Overholt, S
Published in: Anticancer research
January 2008

The effectiveness of photodynamic therapy (PDT) for ablation of high grade dysplasia (HGD) in Barrett's esophagus (BE) is typically reported histologically. Following successful PDT, Barrett's mucosa is replaced with neosquamous mucosa. The objective of this study was to compare the expression of p53 protein in neosquamous mucosa as compared to that in HGD samples not treated with PDT.The patients were divided into two groups. Group I patients (n = 12) had been treated with PDT for HGD and provided 23 biopsy samples of neosquamous mucosa. Group II patients (n = 10) had not received any ablative therapies for BE and provided 14 HGD samples. The immunohistochemical (IHC) staining for p53 protein was performed using mouse anti-human monoclonal antibody DO-1. The degree of p53 protein expression in the cell nuclei was scored using an established IHC scoring system (0 for negative samples and range of 2 to 8 for positive samples).The HGD samples showed diffuse strong p53 staining. The median IHC score for HGD was 7.0. The median IHC score for neosquamous mucosa following PDT was 4.0, with positive scores indicating weak staining in the basal layer of the neosquamous samples. There was significantly lower p53 expression in the neosquamous samples compared to that in the HGD samples (p < 0.001).Significantly lower p53 protein expression was detected in neosquamous mucosa of patients who had received PDT for HGD, suggesting a decreased risk for neoplastic progression after treatment.

Duke Scholars

Published In

Anticancer research

EISSN

1791-7530

ISSN

0250-7005

Publication Date

January 2008

Volume

28

Issue

1B

Start / End Page

485 / 489

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Photochemotherapy
  • Oncology & Carcinogenesis
  • Mucous Membrane
  • Immunohistochemistry
  • Humans
  • Esophagus
  • Barrett Esophagus
  • 3211 Oncology and carcinogenesis
  • 1112 Oncology and Carcinogenesis
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Panjehpour, M., Coppola, D., Overholt, B. F., Vo-Dinh, T., & Overholt, S. (2008). Photodynamic therapy of Barrett's esophagus: ablation of Barrett's mucosa and reduction in p53 protein expression after treatment. Anticancer Research, 28(1B), 485–489.
Panjehpour, Masoud, Domenico Coppola, Bergein F. Overholt, Tuan Vo-Dinh, and Suzanne Overholt. “Photodynamic therapy of Barrett's esophagus: ablation of Barrett's mucosa and reduction in p53 protein expression after treatment.Anticancer Research 28, no. 1B (January 2008): 485–89.
Panjehpour M, Coppola D, Overholt BF, Vo-Dinh T, Overholt S. Photodynamic therapy of Barrett's esophagus: ablation of Barrett's mucosa and reduction in p53 protein expression after treatment. Anticancer research. 2008 Jan;28(1B):485–9.
Panjehpour, Masoud, et al. “Photodynamic therapy of Barrett's esophagus: ablation of Barrett's mucosa and reduction in p53 protein expression after treatment.Anticancer Research, vol. 28, no. 1B, Jan. 2008, pp. 485–89.
Panjehpour M, Coppola D, Overholt BF, Vo-Dinh T, Overholt S. Photodynamic therapy of Barrett's esophagus: ablation of Barrett's mucosa and reduction in p53 protein expression after treatment. Anticancer research. 2008 Jan;28(1B):485–489.

Published In

Anticancer research

EISSN

1791-7530

ISSN

0250-7005

Publication Date

January 2008

Volume

28

Issue

1B

Start / End Page

485 / 489

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Photochemotherapy
  • Oncology & Carcinogenesis
  • Mucous Membrane
  • Immunohistochemistry
  • Humans
  • Esophagus
  • Barrett Esophagus
  • 3211 Oncology and carcinogenesis
  • 1112 Oncology and Carcinogenesis