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Macrophage activation by gastric fluid suggests MMP involvement in aspiration-induced lung disease.

Publication ,  Journal Article
Cheng, CM; Hsieh, CC; Lin, CS; Dai, ZK; Shih, PK; Everett, ML; Thomas, AD; Parker, W; Davis, RD; Lin, SS
Published in: Immunobiology
March 2010

Asthma occurs in more than 5% of the population in industrialized countries and is now characterized as a chronic inflammatory disease. The chronic aspiration of gastric fluid is considered by many investigators to be a primary inflammatory factor exacerbating or predisposing patients to asthma, with more than 50 medical papers per year linking asthma with gastroesophageal reflux disease (GERD), which can lead to aspiration events. However, the mechanisms involved in the inflammatory effects caused by gastric-fluid aspiration are not clear at the present time. The role of macrophages in the pathogenesis of disease seems likely given the involvement of those cells in a variety of chronic inflammatory diseases. To investigate the potential role of gastric fluid and the mechanisms potentially underlying chronic aspiration-associated pathogenesis, we examined the activation of murine macrophages (Raw 264.7 cell line) with gastric fluid. Inflammatory cytokine production and activation of the NF-kappaB signaling pathway were observed. Toll-like receptor (TLR)-4-dependent activation was observed under some conditions, indicating that bacterial components within the gastric fluid are involved in macrophage activation. Matrix metalloproteinase-9 (MMP-9) expression by macrophages was enhanced by gastric fluid, suggesting a potential mechanism by which remodeling of airways might be induced by gastric-fluid aspiration.

Duke Scholars

Published In

Immunobiology

DOI

EISSN

1878-3279

Publication Date

March 2010

Volume

215

Issue

3

Start / End Page

173 / 181

Location

Netherlands

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Signal Transduction
  • NF-kappa B
  • Mice, Inbred BALB C
  • Mice
  • Matrix Metalloproteinases
  • Male
  • Macrophages
  • Macrophage Activation
  • Lung Diseases
 

Citation

APA
Chicago
ICMJE
MLA
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Cheng, C. M., Hsieh, C. C., Lin, C. S., Dai, Z. K., Shih, P. K., Everett, M. L., … Lin, S. S. (2010). Macrophage activation by gastric fluid suggests MMP involvement in aspiration-induced lung disease. Immunobiology, 215(3), 173–181. https://doi.org/10.1016/j.imbio.2009.02.001
Cheng, Chih Mei, Chong Chao Hsieh, Chang Sheng Lin, Zen Kong Dai, Pin Keng Shih, Mary Lou Everett, Anitra D. Thomas, William Parker, R Duane Davis, and Shu S. Lin. “Macrophage activation by gastric fluid suggests MMP involvement in aspiration-induced lung disease.Immunobiology 215, no. 3 (March 2010): 173–81. https://doi.org/10.1016/j.imbio.2009.02.001.
Cheng CM, Hsieh CC, Lin CS, Dai ZK, Shih PK, Everett ML, et al. Macrophage activation by gastric fluid suggests MMP involvement in aspiration-induced lung disease. Immunobiology. 2010 Mar;215(3):173–81.
Cheng, Chih Mei, et al. “Macrophage activation by gastric fluid suggests MMP involvement in aspiration-induced lung disease.Immunobiology, vol. 215, no. 3, Mar. 2010, pp. 173–81. Pubmed, doi:10.1016/j.imbio.2009.02.001.
Cheng CM, Hsieh CC, Lin CS, Dai ZK, Shih PK, Everett ML, Thomas AD, Parker W, Davis RD, Lin SS. Macrophage activation by gastric fluid suggests MMP involvement in aspiration-induced lung disease. Immunobiology. 2010 Mar;215(3):173–181.
Journal cover image

Published In

Immunobiology

DOI

EISSN

1878-3279

Publication Date

March 2010

Volume

215

Issue

3

Start / End Page

173 / 181

Location

Netherlands

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Signal Transduction
  • NF-kappa B
  • Mice, Inbred BALB C
  • Mice
  • Matrix Metalloproteinases
  • Male
  • Macrophages
  • Macrophage Activation
  • Lung Diseases