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12(S)-hydroxy-5,8,10,14-eicosatetraenoic acid is a more potent neutrophil chemoattractant than the 12(R) epimer in the rat cornea.

Publication ,  Journal Article
Wiggins, RE; Jafri, MS; Proia, AD
Published in: Prostaglandins
August 1990

12(R)-hydroxy-5,8,10,14-eicosatetraenoic acid [12(R)-HETE] is reported to be more potent than its epimer 12(S)-HETE as a chemoattractant for human neutrophils in vitro and following topical application to the skin. To assess the in vivo neutrophil chemoattractant potencies of 12(S)-HETE and 12(R)-HETE in the rat, we injected 1 microgram, 5 micrograms, or 10 micrograms of these eicosanoids into the corneal stroma. Rats were killed 12-15 hours after injection, and the number of neutrophils in the stroma was counted in a histological section of the cornea including the injection site. The number of neutrophils was significantly increased in corneas injected with 5 micrograms (+103% of control) or 10 micrograms (+456% of control) of 12(S)-HETE and in those injected with 10 micrograms of 12(R)-HETE (+111% of control). The neutrophilic infiltrate in corneas injected with 1 microgram or 5 micrograms of 12(S)-HETE was not significantly different from that in corneas injected with 1 microgram of leukotriene B4. The data for the 10 micrograms injections indicate that 12(S)-HETE is a more potent neutrophil chemoattractant than 12(R)-HETE in the rat cornea. Our results suggest that species or tissue specificity may determine the relative potencies of 12-HETE epimers as chemoattractants for neutrophils, and that 12(S)-HETE may be an important inflammatory mediator in the rat cornea.

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Published In

Prostaglandins

DOI

ISSN

0090-6980

Publication Date

August 1990

Volume

40

Issue

2

Start / End Page

131 / 141

Location

United States

Related Subject Headings

  • Stereoisomerism
  • Rats, Inbred Strains
  • Rats
  • Neutrophils
  • Leukotriene B4
  • Hydroxyeicosatetraenoic Acids
  • Epithelium
  • Dimethyl Sulfoxide
  • Cornea
  • Chemotaxis, Leukocyte
 

Citation

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Wiggins, R. E., Jafri, M. S., & Proia, A. D. (1990). 12(S)-hydroxy-5,8,10,14-eicosatetraenoic acid is a more potent neutrophil chemoattractant than the 12(R) epimer in the rat cornea. Prostaglandins, 40(2), 131–141. https://doi.org/10.1016/0090-6980(90)90079-b
Wiggins, R. E., M. S. Jafri, and A. D. Proia. “12(S)-hydroxy-5,8,10,14-eicosatetraenoic acid is a more potent neutrophil chemoattractant than the 12(R) epimer in the rat cornea.Prostaglandins 40, no. 2 (August 1990): 131–41. https://doi.org/10.1016/0090-6980(90)90079-b.
Wiggins, R. E., et al. “12(S)-hydroxy-5,8,10,14-eicosatetraenoic acid is a more potent neutrophil chemoattractant than the 12(R) epimer in the rat cornea.Prostaglandins, vol. 40, no. 2, Aug. 1990, pp. 131–41. Pubmed, doi:10.1016/0090-6980(90)90079-b.

Published In

Prostaglandins

DOI

ISSN

0090-6980

Publication Date

August 1990

Volume

40

Issue

2

Start / End Page

131 / 141

Location

United States

Related Subject Headings

  • Stereoisomerism
  • Rats, Inbred Strains
  • Rats
  • Neutrophils
  • Leukotriene B4
  • Hydroxyeicosatetraenoic Acids
  • Epithelium
  • Dimethyl Sulfoxide
  • Cornea
  • Chemotaxis, Leukocyte