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Qa-1, a nonclassical MHC molecule with immunomodulatory functions, is ubiquitously expressed in the immune-privileged anterior chamber of the eye.

Publication ,  Journal Article
Kim, CY; Masli, S; Streilein, JW
Published in: Ocul Immunol Inflamm
2005

PURPOSE: To determine whether the MHC class Ib gene, Qa-1, is expressed in the tissues that surround the immune-privileged anterior chamber (AC) of the murine eye. METHODS: Transcription of Qa-1 mRNA in BALB/c ocular tissues was analyzed by reverse transcription-polymerase chain reaction. Expression of Qa-1 protein was assessed on ocular frozen tissue sections by immunohistochemistry, and within aqueous humor by western blotting. RESULTS: Transcription of Qa-1 was found in all tissues surrounding the AC of the eye. Immunohistological staining revealed Qa-1 expression on corneal endothelium, corneal epithelium, and lens epithelium. No soluble Qa-1 was detected in aqueous humor. CONCLUSIONS: Qa-1, unlike other MHC class I molecules, is ubiquitously expressed in tissues surrounding the AC of the eye, raising the possibility that Qa-1 plays a role in creating and maintaining ocular immune privilege.

Duke Scholars

Published In

Ocul Immunol Inflamm

DOI

ISSN

0927-3948

Publication Date

2005

Volume

13

Issue

4

Start / End Page

271 / 277

Location

England

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Ophthalmology & Optometry
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Lens, Crystalline
  • Iris
  • Immunohistochemistry
  • Histocompatibility Antigens Class I
 

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Kim, C. Y., Masli, S., & Streilein, J. W. (2005). Qa-1, a nonclassical MHC molecule with immunomodulatory functions, is ubiquitously expressed in the immune-privileged anterior chamber of the eye. Ocul Immunol Inflamm, 13(4), 271–277. https://doi.org/10.1080/09273940590951052
Kim, Charles Y., Sharmila Masli, and J Wayne Streilein. “Qa-1, a nonclassical MHC molecule with immunomodulatory functions, is ubiquitously expressed in the immune-privileged anterior chamber of the eye.Ocul Immunol Inflamm 13, no. 4 (2005): 271–77. https://doi.org/10.1080/09273940590951052.
Kim, Charles Y., et al. “Qa-1, a nonclassical MHC molecule with immunomodulatory functions, is ubiquitously expressed in the immune-privileged anterior chamber of the eye.Ocul Immunol Inflamm, vol. 13, no. 4, 2005, pp. 271–77. Pubmed, doi:10.1080/09273940590951052.
Journal cover image

Published In

Ocul Immunol Inflamm

DOI

ISSN

0927-3948

Publication Date

2005

Volume

13

Issue

4

Start / End Page

271 / 277

Location

England

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Ophthalmology & Optometry
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Lens, Crystalline
  • Iris
  • Immunohistochemistry
  • Histocompatibility Antigens Class I