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Molecular analysis of HLA-B35 alleles and their relationship to HLA-B15 alleles.

Publication ,  Journal Article
Cereb, N; Kim, C; Hughes, AL; Yang, SY
Published in: Tissue Antigens
April 1997

The HLA-B35 serotype is one of the largest allelic groups of HLA class I molecules and includes four isotypes. Of the four, the B35 variant isoform is relatively rare and is the most acidic form. DNA sequencing of the rare isoforms revealed three alleles, B*1522, B*3511, and B*3517. A phylogenetic tree of HLA-B15- and HLA-B35-related alleles for the exon 2 and 3 nucleotide sequences showed that exon 2 of B*1522 clusters with B35 alleles whereas exon 3 clusters with B15 alleles. Branches of the tree suggest that the serodeterminants of B35, B62, B63, and B70 may reside in the alpha 1 domain, encoded by exon 2. The B*1520 and B*1522 genes, which type as B62 and B35, respectively, are hybrid molecules alternatively using exon 2 and exon 3 sequences of B*3501 and B*1501. A comparison of intron 2 sequences for B*3501, B*1501 and B*1522 suggests that the recombination site may have been in the region at the 3' end of intron 2. Despite being flanked by two highly polymorphic exons (exons 2 and 3), intron 2 is relatively well conserved in the B-locus, and it is characterized by seven to eight tandem repeats of the CGGGG pentanucleotide. A high degree of sequence homology and repetitive sequences are essential for a significant frequency of recombination. In this report, we reveal more about the complex evolutionary history of the HLA-B alleles.

Duke Scholars

Published In

Tissue Antigens

DOI

ISSN

0001-2815

Publication Date

April 1997

Volume

49

Issue

4

Start / End Page

389 / 396

Location

England

Related Subject Headings

  • Sequence Analysis, DNA
  • Sequence Alignment
  • Molecular Sequence Data
  • Immunology
  • Humans
  • HLA-B35 Antigen
  • HLA-B15 Antigen
  • HLA-B Antigens
  • Evolution, Molecular
  • Base Sequence
 

Citation

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Cereb, N., Kim, C., Hughes, A. L., & Yang, S. Y. (1997). Molecular analysis of HLA-B35 alleles and their relationship to HLA-B15 alleles. Tissue Antigens, 49(4), 389–396. https://doi.org/10.1111/j.1399-0039.1997.tb02767.x
Cereb, N., C. Kim, A. L. Hughes, and S. Y. Yang. “Molecular analysis of HLA-B35 alleles and their relationship to HLA-B15 alleles.Tissue Antigens 49, no. 4 (April 1997): 389–96. https://doi.org/10.1111/j.1399-0039.1997.tb02767.x.
Cereb N, Kim C, Hughes AL, Yang SY. Molecular analysis of HLA-B35 alleles and their relationship to HLA-B15 alleles. Tissue Antigens. 1997 Apr;49(4):389–96.
Cereb, N., et al. “Molecular analysis of HLA-B35 alleles and their relationship to HLA-B15 alleles.Tissue Antigens, vol. 49, no. 4, Apr. 1997, pp. 389–96. Pubmed, doi:10.1111/j.1399-0039.1997.tb02767.x.
Cereb N, Kim C, Hughes AL, Yang SY. Molecular analysis of HLA-B35 alleles and their relationship to HLA-B15 alleles. Tissue Antigens. 1997 Apr;49(4):389–396.
Journal cover image

Published In

Tissue Antigens

DOI

ISSN

0001-2815

Publication Date

April 1997

Volume

49

Issue

4

Start / End Page

389 / 396

Location

England

Related Subject Headings

  • Sequence Analysis, DNA
  • Sequence Alignment
  • Molecular Sequence Data
  • Immunology
  • Humans
  • HLA-B35 Antigen
  • HLA-B15 Antigen
  • HLA-B Antigens
  • Evolution, Molecular
  • Base Sequence