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CD10/neutral endopeptidase 24.11 regulates fetal lung growth and maturation in utero by potentiating endogenous bombesin-like peptides.

Publication ,  Journal Article
King, KA; Hua, J; Torday, JS; Drazen, JM; Graham, SA; Shipp, MA; Sunday, ME
Published in: J Clin Invest
May 1993

Bombesin-like peptides (BLPs) are mitogens for bronchial epithelial cells and small cell lung carcinomas, and increase fetal lung growth and maturation in utero and in organ cultures. BLPs are hydrolyzed by the enzyme CD10/neutral endopeptidase 24.11 (CD10/NEP) which is expressed in bronchial epithelium and functions to inhibit BLP-mediated growth of small cell lung carcinomas. To determine whether CD10/NEP regulates peptide-mediated lung development, we administered a specific CD10/NEP inhibitor, SCH32615, to fetal mice in utero from gestational days e15-17. Fetal lung tissues were evaluated on e18 for: (a) growth using [3H]thymidine incorporation into nuclear DNA; and (b) maturation using: [3H]-choline incorporation into surfactant phospholipids, electron microscopy for type II pneumocytes, and Northern blot analyses for surfactant apoproteins A, B, and C. Inhibition of CD10/NEP stimulated [3H]thymidine incorporation into DNA (70% above baseline, P < 0.005), [3H]choline incorporation into surfactant phospholipids (38% above baseline, P < 0.005), increased numbers of type II pneumocytes (36% above baseline, P = 0.07), and fivefold higher surfactant protein A transcripts (P < 0.05). CD10/NEP-mediated effects were completely blocked by the specific bombesin receptor antagonist, [D-Phe12, Leu14]bombesin. These observations suggest that CD10/NEP regulates fetal lung growth and maturation mediated by endogenous BLPs.

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Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

May 1993

Volume

91

Issue

5

Start / End Page

1969 / 1973

Location

United States

Related Subject Headings

  • Thymidine
  • Proteins
  • Pregnancy
  • Phosphatidylcholines
  • Neprilysin
  • Mice
  • Lung
  • Kinetics
  • Immunology
  • Fetus
 

Citation

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King, K. A., Hua, J., Torday, J. S., Drazen, J. M., Graham, S. A., Shipp, M. A., & Sunday, M. E. (1993). CD10/neutral endopeptidase 24.11 regulates fetal lung growth and maturation in utero by potentiating endogenous bombesin-like peptides. J Clin Invest, 91(5), 1969–1973. https://doi.org/10.1172/JCI116417
King, K. A., J. Hua, J. S. Torday, J. M. Drazen, S. A. Graham, M. A. Shipp, and M. E. Sunday. “CD10/neutral endopeptidase 24.11 regulates fetal lung growth and maturation in utero by potentiating endogenous bombesin-like peptides.J Clin Invest 91, no. 5 (May 1993): 1969–73. https://doi.org/10.1172/JCI116417.
King KA, Hua J, Torday JS, Drazen JM, Graham SA, Shipp MA, et al. CD10/neutral endopeptidase 24.11 regulates fetal lung growth and maturation in utero by potentiating endogenous bombesin-like peptides. J Clin Invest. 1993 May;91(5):1969–73.
King, K. A., et al. “CD10/neutral endopeptidase 24.11 regulates fetal lung growth and maturation in utero by potentiating endogenous bombesin-like peptides.J Clin Invest, vol. 91, no. 5, May 1993, pp. 1969–73. Pubmed, doi:10.1172/JCI116417.
King KA, Hua J, Torday JS, Drazen JM, Graham SA, Shipp MA, Sunday ME. CD10/neutral endopeptidase 24.11 regulates fetal lung growth and maturation in utero by potentiating endogenous bombesin-like peptides. J Clin Invest. 1993 May;91(5):1969–1973.

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

May 1993

Volume

91

Issue

5

Start / End Page

1969 / 1973

Location

United States

Related Subject Headings

  • Thymidine
  • Proteins
  • Pregnancy
  • Phosphatidylcholines
  • Neprilysin
  • Mice
  • Lung
  • Kinetics
  • Immunology
  • Fetus