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Transient elevation of messenger RNA encoding gastrin-releasing peptide, a putative pulmonary growth factor in human fetal lung.

Publication ,  Journal Article
Spindel, ER; Sunday, ME; Hofler, H; Wolfe, HJ; Habener, JF; Chin, WW
Published in: J Clin Invest
October 1987

Gastrin-releasing peptide (GRP), the mammalian homologue of the amphibian peptide bombesin, is present in pulmonary neuroendocrine cells and appears to be a growth factor for both normal and neoplastic pulmonary cells. Previously we have reported the cloning of the messenger RNAs (mRNAs) and gene that encode human GRP. We now report that GRP mRNAs are markedly elevated in human fetal lung during the canalicular phase of pulmonary development (from approximately 16 to 30 wk gestation). By RNA blot and in situ hybridization analyses, GRP mRNAs were first detectable in fetal lung at 9-10 wk, plateaued at levels 25-fold higher than in adult lungs from 16 to approximately 30 wk and then declined to near adult levels by 34 wk gestation. By contrast, GRP peptide levels remain elevated until several months after birth. Consistent with this, in situ hybridization and immunohistochemical studies showed that GRP mRNA and peptide consistently colocalized in early gestation lung but that in neonatal lung, many cells that contained GRP peptide no longer contained GRP mRNA. The transient expression of high levels of GRP mRNAs during an approximately 12-wk phase of fetal lung development suggests that the secretion of GRP or its COOH-terminal peptides from pulmonary neuroendocrine cells may play a role in normal lung development.

Duke Scholars

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

October 1987

Volume

80

Issue

4

Start / End Page

1172 / 1179

Location

United States

Related Subject Headings

  • RNA, Messenger
  • Peptides
  • Nucleic Acid Hybridization
  • Lung
  • Immunology
  • Immunohistochemistry
  • Humans
  • Gestational Age
  • Gastrin-Releasing Peptide
  • Embryonic and Fetal Development
 

Citation

APA
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MLA
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Spindel, E. R., Sunday, M. E., Hofler, H., Wolfe, H. J., Habener, J. F., & Chin, W. W. (1987). Transient elevation of messenger RNA encoding gastrin-releasing peptide, a putative pulmonary growth factor in human fetal lung. J Clin Invest, 80(4), 1172–1179. https://doi.org/10.1172/JCI113176
Spindel, E. R., M. E. Sunday, H. Hofler, H. J. Wolfe, J. F. Habener, and W. W. Chin. “Transient elevation of messenger RNA encoding gastrin-releasing peptide, a putative pulmonary growth factor in human fetal lung.J Clin Invest 80, no. 4 (October 1987): 1172–79. https://doi.org/10.1172/JCI113176.
Spindel ER, Sunday ME, Hofler H, Wolfe HJ, Habener JF, Chin WW. Transient elevation of messenger RNA encoding gastrin-releasing peptide, a putative pulmonary growth factor in human fetal lung. J Clin Invest. 1987 Oct;80(4):1172–9.
Spindel, E. R., et al. “Transient elevation of messenger RNA encoding gastrin-releasing peptide, a putative pulmonary growth factor in human fetal lung.J Clin Invest, vol. 80, no. 4, Oct. 1987, pp. 1172–79. Pubmed, doi:10.1172/JCI113176.
Spindel ER, Sunday ME, Hofler H, Wolfe HJ, Habener JF, Chin WW. Transient elevation of messenger RNA encoding gastrin-releasing peptide, a putative pulmonary growth factor in human fetal lung. J Clin Invest. 1987 Oct;80(4):1172–1179.

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

October 1987

Volume

80

Issue

4

Start / End Page

1172 / 1179

Location

United States

Related Subject Headings

  • RNA, Messenger
  • Peptides
  • Nucleic Acid Hybridization
  • Lung
  • Immunology
  • Immunohistochemistry
  • Humans
  • Gestational Age
  • Gastrin-Releasing Peptide
  • Embryonic and Fetal Development