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Generation and characterization of mice lacking gastrin-releasing peptide receptor.

Publication ,  Journal Article
Wada, E; Watase, K; Yamada, K; Ogura, H; Yamano, M; Inomata, Y; Eguchi, J; Yamamoto, K; Sunday, ME; Maeno, H; Mikoshiba, K; Ohki-Hamazaki, H; Wada, K
Published in: Biochem Biophys Res Commun
October 9, 1997

Gastrin-releasing peptide (GRP) is a mammalian bombesin-like peptide which is widely distributed in the central nervous system as well as in the gastrointestinal tract. GRP binds to its high affinity receptor (GRPR) to elicit a wide spectrum of biological effects on behavior, digestion, and metabolism. To define the in vivo function of GRPR, we generated GRPR null mutant mice by gene targeting. The intracerebroventricular administration of GRP caused hypothermia in wild-type mice, but not in mutant mice. The GRPR deficient mice showed significantly increased locomotor activity during the dark period, and social responses scored by sniffing, mounting, and approaching behaviors against an intruder. Aggressive scores such as fighting and biting were not altered in the mutant mice. These phenotypes were observed in mice generated from two independent ES cell clones and backcrossed to a C57BL/6J background. The GRPR deficient mice should be useful for studying the bombesin system in vivo.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

October 9, 1997

Volume

239

Issue

1

Start / End Page

28 / 33

Location

United States

Related Subject Headings

  • Rett Syndrome
  • Receptors, Bombesin
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
  • Gene Targeting
  • Brain
  • Biogenic Monoamines
  • Biochemistry & Molecular Biology
  • Animals
 

Citation

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Wada, E., Watase, K., Yamada, K., Ogura, H., Yamano, M., Inomata, Y., … Wada, K. (1997). Generation and characterization of mice lacking gastrin-releasing peptide receptor. Biochem Biophys Res Commun, 239(1), 28–33. https://doi.org/10.1006/bbrc.1997.7418
Wada, E., K. Watase, K. Yamada, H. Ogura, M. Yamano, Y. Inomata, J. Eguchi, et al. “Generation and characterization of mice lacking gastrin-releasing peptide receptor.Biochem Biophys Res Commun 239, no. 1 (October 9, 1997): 28–33. https://doi.org/10.1006/bbrc.1997.7418.
Wada E, Watase K, Yamada K, Ogura H, Yamano M, Inomata Y, et al. Generation and characterization of mice lacking gastrin-releasing peptide receptor. Biochem Biophys Res Commun. 1997 Oct 9;239(1):28–33.
Wada, E., et al. “Generation and characterization of mice lacking gastrin-releasing peptide receptor.Biochem Biophys Res Commun, vol. 239, no. 1, Oct. 1997, pp. 28–33. Pubmed, doi:10.1006/bbrc.1997.7418.
Wada E, Watase K, Yamada K, Ogura H, Yamano M, Inomata Y, Eguchi J, Yamamoto K, Sunday ME, Maeno H, Mikoshiba K, Ohki-Hamazaki H, Wada K. Generation and characterization of mice lacking gastrin-releasing peptide receptor. Biochem Biophys Res Commun. 1997 Oct 9;239(1):28–33.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

October 9, 1997

Volume

239

Issue

1

Start / End Page

28 / 33

Location

United States

Related Subject Headings

  • Rett Syndrome
  • Receptors, Bombesin
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
  • Gene Targeting
  • Brain
  • Biogenic Monoamines
  • Biochemistry & Molecular Biology
  • Animals