Detection of Pneumocystis jiroveci in respiratory specimens by four staining methods.
Publication
, Journal Article
Procop, GW; Haddad, S; Quinn, J; Wilson, ML; Henshaw, NG; Reller, LB; Artymyshyn, RL; Katanik, MT; Weinstein, MP
Published in: J Clin Microbiol
July 2004
We examined four staining methods on replicate smears of 313 respiratory specimens submitted for Pneumocystis jiroveci examination. The sensitivity and specificity of Calcofluor white stain (CW) were 73.8 and 99.6%, respectively. The sensitivity and specificity of Grocott-Gomori methenamine silver stain (GMS) were 79.4 and 99.2%, respectively. The sensitivity and specificity of Diff-Quik stain were 49.2 and 99.6%, respectively. The sensitivity and specificity of Merifluor Pneumocystis stain were 90.8 and 81.9%, respectively. Only CW and GMS had positive and negative predictive values of >90%.
Duke Scholars
Altmetric Attention Stats
Dimensions Citation Stats
Published In
J Clin Microbiol
DOI
ISSN
0095-1137
Publication Date
July 2004
Volume
42
Issue
7
Start / End Page
3333 / 3335
Location
United States
Related Subject Headings
- Staining and Labeling
- Sputum
- Sensitivity and Specificity
- Pneumocystis carinii
- Microbiology
- Humans
- Bronchoalveolar Lavage Fluid
- 3207 Medical microbiology
- 3202 Clinical sciences
- 11 Medical and Health Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Procop, G. W., Haddad, S., Quinn, J., Wilson, M. L., Henshaw, N. G., Reller, L. B., … Weinstein, M. P. (2004). Detection of Pneumocystis jiroveci in respiratory specimens by four staining methods. J Clin Microbiol, 42(7), 3333–3335. https://doi.org/10.1128/JCM.42.7.3333-3335.2004
Procop, G. W., S. Haddad, J. Quinn, M. L. Wilson, N. G. Henshaw, L. B. Reller, R. L. Artymyshyn, M. T. Katanik, and M. P. Weinstein. “Detection of Pneumocystis jiroveci in respiratory specimens by four staining methods.” J Clin Microbiol 42, no. 7 (July 2004): 3333–35. https://doi.org/10.1128/JCM.42.7.3333-3335.2004.
Procop GW, Haddad S, Quinn J, Wilson ML, Henshaw NG, Reller LB, et al. Detection of Pneumocystis jiroveci in respiratory specimens by four staining methods. J Clin Microbiol. 2004 Jul;42(7):3333–5.
Procop, G. W., et al. “Detection of Pneumocystis jiroveci in respiratory specimens by four staining methods.” J Clin Microbiol, vol. 42, no. 7, July 2004, pp. 3333–35. Pubmed, doi:10.1128/JCM.42.7.3333-3335.2004.
Procop GW, Haddad S, Quinn J, Wilson ML, Henshaw NG, Reller LB, Artymyshyn RL, Katanik MT, Weinstein MP. Detection of Pneumocystis jiroveci in respiratory specimens by four staining methods. J Clin Microbiol. 2004 Jul;42(7):3333–3335.
Published In
J Clin Microbiol
DOI
ISSN
0095-1137
Publication Date
July 2004
Volume
42
Issue
7
Start / End Page
3333 / 3335
Location
United States
Related Subject Headings
- Staining and Labeling
- Sputum
- Sensitivity and Specificity
- Pneumocystis carinii
- Microbiology
- Humans
- Bronchoalveolar Lavage Fluid
- 3207 Medical microbiology
- 3202 Clinical sciences
- 11 Medical and Health Sciences