Detection of humoral response in patients with glioblastoma receiving EGFRvIII-KLH vaccines.
The epidermal growth factor receptor variant III (EGFRvIII) is a consistent tumor-specific mutation that is widely expressed in glioblastoma multiforme (GBM) and other neoplasms. As such it represents a truly tumor-specific target for antitumor immunotherapy. Although endogenous humoral responses to EGFRvIII have been reported in patients with EGFRvIII-expressing breast cancer, it is not known whether de novo responses can be generated or endogenous responses enhanced with an EGFRvIII-specific vaccine. To assess this in clinical trials, we have developed and validated an immunoassay to measure and isolate anti-EGFRvIII and anti-KLH antibodies from the serum of patients vaccinated with an EGFRvIII-specific peptide (PEPvIII) conjugated to keyhole limpet hemocyanin (KLH). Using magnetic beads with immobilized antigen we captured and detected anti-EGFRvIII and anti-KLH antibodies in serum from patients before and after vaccinations. Using this assay, we found that significant levels of antibody for tumor-specific antigen EGFRvIII (>4 microg/mL) and KLH could be induced after vaccination with PEPvIII-KLH.
Duke Scholars
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Related Subject Headings
- Vaccination
- Receptor, Fibroblast Growth Factor, Type 3
- Male
- Immunology
- Humans
- Hemocyanins
- Glioblastoma
- Female
- Cancer Vaccines
- Antibody Formation
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Vaccination
- Receptor, Fibroblast Growth Factor, Type 3
- Male
- Immunology
- Humans
- Hemocyanins
- Glioblastoma
- Female
- Cancer Vaccines
- Antibody Formation