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Efficient dendritic cell priming of T lymphocytes depends on the extracellular matrix protein mindin.

Publication ,  Journal Article
Li, H; Oliver, T; Jia, W; He, Y-W
Published in: EMBO J
September 6, 2006

Rho guanosine triphosphatases (GTPases) regulate multiple aspects of dendritic cell (DC) function, but what regulates the expression of Rho GTPases in DCs is unknown. Here, we show that the extracellular matrix protein mindin regulates the expression of Rho GTPases in DCs. Mindin(-/-) mice displayed defective CD4+ T-cell priming and impaired humoral immune responses to T-dependent antigens. Mindin(-/-) DCs had reduced expression of Rac1/2 and impaired priming capacity owing to inefficient engagement with T lymphocytes. Ectopic Rac1 expression restored the priming capability of Mindin(-/-) DCs. Furthermore, we show that DC adhesion to mindin matrix was blocked by antibodies to alpha4, alpha5 and beta1 integrins. DCs lacking beta1 integrin had reduced adhesion to mindin matrix, decreased expression of Rac1/2 and impaired priming capacity. These results suggest that mindin-integrin interactions play a key role in regulating Rho GTPase expression in DCs and DC priming of T lymphocytes.

Duke Scholars

Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

September 6, 2006

Volume

25

Issue

17

Start / End Page

4097 / 4107

Location

England

Related Subject Headings

  • rho GTP-Binding Proteins
  • rac1 GTP-Binding Protein
  • rac GTP-Binding Proteins
  • RAC2 GTP-Binding Protein
  • Mice, Knockout
  • Mice
  • Integrins
  • Gene Expression Regulation
  • Extracellular Matrix Proteins
  • Developmental Biology
 

Citation

APA
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MLA
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Li, H., Oliver, T., Jia, W., & He, Y.-W. (2006). Efficient dendritic cell priming of T lymphocytes depends on the extracellular matrix protein mindin. EMBO J, 25(17), 4097–4107. https://doi.org/10.1038/sj.emboj.7601289
Li, Hong, Tim Oliver, Wei Jia, and You-Wen He. “Efficient dendritic cell priming of T lymphocytes depends on the extracellular matrix protein mindin.EMBO J 25, no. 17 (September 6, 2006): 4097–4107. https://doi.org/10.1038/sj.emboj.7601289.
Li H, Oliver T, Jia W, He Y-W. Efficient dendritic cell priming of T lymphocytes depends on the extracellular matrix protein mindin. EMBO J. 2006 Sep 6;25(17):4097–107.
Li, Hong, et al. “Efficient dendritic cell priming of T lymphocytes depends on the extracellular matrix protein mindin.EMBO J, vol. 25, no. 17, Sept. 2006, pp. 4097–107. Pubmed, doi:10.1038/sj.emboj.7601289.
Li H, Oliver T, Jia W, He Y-W. Efficient dendritic cell priming of T lymphocytes depends on the extracellular matrix protein mindin. EMBO J. 2006 Sep 6;25(17):4097–4107.

Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

September 6, 2006

Volume

25

Issue

17

Start / End Page

4097 / 4107

Location

England

Related Subject Headings

  • rho GTP-Binding Proteins
  • rac1 GTP-Binding Protein
  • rac GTP-Binding Proteins
  • RAC2 GTP-Binding Protein
  • Mice, Knockout
  • Mice
  • Integrins
  • Gene Expression Regulation
  • Extracellular Matrix Proteins
  • Developmental Biology