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Arterial pole progenitors interpret opposing FGF/BMP signals to proliferate or differentiate.

Publication ,  Journal Article
Hutson, MR; Zeng, XL; Kim, AJ; Antoon, E; Harward, S; Kirby, ML
Published in: Development
September 2010

During heart development, a subpopulation of cells in the heart field maintains cardiac potential over several days of development and forms the myocardium and smooth muscle of the arterial pole. Using clonal and explant culture experiments, we show that these cells are a stem cell population that can differentiate into myocardium, smooth muscle and endothelial cells. The multipotent stem cells proliferate or differentiate into different cardiovascular cell fates through activation or inhibition of FGF and BMP signaling pathways. BMP promoted myocardial differentiation but not proliferation. FGF signaling promoted proliferation and induced smooth muscle differentiation, but inhibited myocardial differentiation. Blocking the Ras/Erk intracellular pathway promoted myocardial differentiation, while the PLCgamma and PI3K pathways regulated proliferation. In vivo, inhibition of both pathways resulted in predictable arterial pole defects. These studies suggest that myocardial differentiation of arterial pole progenitors requires BMP signaling combined with downregulation of the FGF/Ras/Erk pathway. The FGF pathway maintains the pool of proliferating stem cells and later promotes smooth muscle differentiation.

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Published In

Development

DOI

EISSN

1477-9129

Publication Date

September 2010

Volume

137

Issue

18

Start / End Page

3001 / 3011

Location

England

Related Subject Headings

  • Tissue Culture Techniques
  • Stem Cells
  • Quail
  • Myocardium
  • Muscle, Smooth
  • MAP Kinase Signaling System
  • Heart
  • Gene Expression Regulation, Developmental
  • Fibroblast Growth Factor 8
  • Chick Embryo
 

Citation

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Hutson, M. R., Zeng, X. L., Kim, A. J., Antoon, E., Harward, S., & Kirby, M. L. (2010). Arterial pole progenitors interpret opposing FGF/BMP signals to proliferate or differentiate. Development, 137(18), 3001–3011. https://doi.org/10.1242/dev.051565
Hutson, Mary Redmond, Xiaopei Lily Zeng, Andrew J. Kim, Emily Antoon, Stephen Harward, and Margaret L. Kirby. “Arterial pole progenitors interpret opposing FGF/BMP signals to proliferate or differentiate.Development 137, no. 18 (September 2010): 3001–11. https://doi.org/10.1242/dev.051565.
Hutson MR, Zeng XL, Kim AJ, Antoon E, Harward S, Kirby ML. Arterial pole progenitors interpret opposing FGF/BMP signals to proliferate or differentiate. Development. 2010 Sep;137(18):3001–11.
Hutson, Mary Redmond, et al. “Arterial pole progenitors interpret opposing FGF/BMP signals to proliferate or differentiate.Development, vol. 137, no. 18, Sept. 2010, pp. 3001–11. Pubmed, doi:10.1242/dev.051565.
Hutson MR, Zeng XL, Kim AJ, Antoon E, Harward S, Kirby ML. Arterial pole progenitors interpret opposing FGF/BMP signals to proliferate or differentiate. Development. 2010 Sep;137(18):3001–3011.
Journal cover image

Published In

Development

DOI

EISSN

1477-9129

Publication Date

September 2010

Volume

137

Issue

18

Start / End Page

3001 / 3011

Location

England

Related Subject Headings

  • Tissue Culture Techniques
  • Stem Cells
  • Quail
  • Myocardium
  • Muscle, Smooth
  • MAP Kinase Signaling System
  • Heart
  • Gene Expression Regulation, Developmental
  • Fibroblast Growth Factor 8
  • Chick Embryo