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Independent and cooperative roles of adaptor molecules in proximal signaling during FcepsilonRI-mediated mast cell activation.

Publication ,  Journal Article
Kambayashi, T; Okumura, M; Baker, RG; Hsu, C-J; Baumgart, T; Zhang, W; Koretzky, GA
Published in: Mol Cell Biol
September 2010

Activation through FcepsilonRI, a high-affinity IgE-binding receptor, is critical for mast cell function during allergy. The formation of a multimolecular proximal signaling complex nucleated by the adaptor molecules SLP-76 and LAT1 is required for activation through this receptor. Based on previous T-cell studies, current dogma dictates that LAT1 is required for plasma membrane recruitment and function of SLP-76. Unexpectedly, we found that the recruitment and phosphorylation of SLP-76 were preserved in LAT1(-/-) mast cells and that SLP-76(-/-) and LAT1(-/-) mast cells harbored distinct functional and biochemical defects. The LAT1-like molecule LAT2 was responsible for the preserved membrane localization and phosphorylation of SLP-76 in LAT1(-/-) mast cells. Although LAT2 supported SLP-76 phosphorylation and recruitment to the plasma membrane, LAT2 only partially compensated for LAT1-mediated cell signaling due to its decreased ability to stabilize interactions with phospholipase Cgamma (PLCgamma). Comparison of SLP-76(-/-) LAT1(-/-) and SLP-76(-/-) mast cells revealed that some functions of LAT1 could occur independently of SLP-76. We propose that while SLP-76 and LAT1 depend on each other for many of their functions, LAT2/SLP-76 interactions and SLP-76-independent LAT1 functions also mediate a positive signaling pathway downstream of FcepsilonRI in mast cells.

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Published In

Mol Cell Biol

DOI

EISSN

1098-5549

Publication Date

September 2010

Volume

30

Issue

17

Start / End Page

4188 / 4196

Location

United States

Related Subject Headings

  • Receptors, IgE
  • Protein Transport
  • Phosphorylation
  • Phosphoproteins
  • Mice
  • Mast Cells
  • Gene Deletion
  • Fusion Regulatory Protein 1, Light Chains
  • Developmental Biology
  • Cells, Cultured
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kambayashi, T., Okumura, M., Baker, R. G., Hsu, C.-J., Baumgart, T., Zhang, W., & Koretzky, G. A. (2010). Independent and cooperative roles of adaptor molecules in proximal signaling during FcepsilonRI-mediated mast cell activation. Mol Cell Biol, 30(17), 4188–4196. https://doi.org/10.1128/MCB.00305-10
Kambayashi, Taku, Mariko Okumura, Rebecca G. Baker, Chih-Jung Hsu, Tobias Baumgart, Weiguo Zhang, and Gary A. Koretzky. “Independent and cooperative roles of adaptor molecules in proximal signaling during FcepsilonRI-mediated mast cell activation.Mol Cell Biol 30, no. 17 (September 2010): 4188–96. https://doi.org/10.1128/MCB.00305-10.
Kambayashi T, Okumura M, Baker RG, Hsu C-J, Baumgart T, Zhang W, et al. Independent and cooperative roles of adaptor molecules in proximal signaling during FcepsilonRI-mediated mast cell activation. Mol Cell Biol. 2010 Sep;30(17):4188–96.
Kambayashi, Taku, et al. “Independent and cooperative roles of adaptor molecules in proximal signaling during FcepsilonRI-mediated mast cell activation.Mol Cell Biol, vol. 30, no. 17, Sept. 2010, pp. 4188–96. Pubmed, doi:10.1128/MCB.00305-10.
Kambayashi T, Okumura M, Baker RG, Hsu C-J, Baumgart T, Zhang W, Koretzky GA. Independent and cooperative roles of adaptor molecules in proximal signaling during FcepsilonRI-mediated mast cell activation. Mol Cell Biol. 2010 Sep;30(17):4188–4196.

Published In

Mol Cell Biol

DOI

EISSN

1098-5549

Publication Date

September 2010

Volume

30

Issue

17

Start / End Page

4188 / 4196

Location

United States

Related Subject Headings

  • Receptors, IgE
  • Protein Transport
  • Phosphorylation
  • Phosphoproteins
  • Mice
  • Mast Cells
  • Gene Deletion
  • Fusion Regulatory Protein 1, Light Chains
  • Developmental Biology
  • Cells, Cultured