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Sources of variability in determining malaria parasite density by microscopy.

Publication ,  Journal Article
O'Meara, WP; McKenzie, FE; Magill, AJ; Forney, JR; Permpanich, B; Lucas, C; Gasser, RA; Wongsrichanalai, C
Published in: Am J Trop Med Hyg
September 2005

Enumeration of parasites by microscopic examination of blood smears is the only method available for quantifying parasitemia in infected blood. However, the sources and scale of error inherent in this technique have not been systematically investigated. Here we use data collected in outpatient clinics in Peru and Thailand to elucidate important sources of variation in parasite density measurements. We show that discrepancies between readings from two independent microscopists and multiple readings from a single microscopist are inversely related to the density of the infection. We present an example of how differences in reader technique, specifically the number of white blood cells counted, can contribute to the differences between readings. We discuss the implications of this analysis for field studies and clinical trials.

Duke Scholars

Published In

Am J Trop Med Hyg

ISSN

0002-9637

Publication Date

September 2005

Volume

73

Issue

3

Start / End Page

593 / 598

Location

United States

Related Subject Headings

  • Tropical Medicine
  • Thailand
  • Peru
  • Observer Variation
  • Microscopy
  • Malaria, Vivax
  • Malaria, Falciparum
  • Leukocytes
  • Humans
  • Animals
 

Citation

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Chicago
ICMJE
MLA
NLM
O’Meara, W. P., McKenzie, F. E., Magill, A. J., Forney, J. R., Permpanich, B., Lucas, C., … Wongsrichanalai, C. (2005). Sources of variability in determining malaria parasite density by microscopy. Am J Trop Med Hyg, 73(3), 593–598.
O’Meara, Wendy Prudhomme, F Ellis McKenzie, Alan J. Magill, J Russ Forney, Barnyen Permpanich, Carmen Lucas, Robert A. Gasser, and Chansuda Wongsrichanalai. “Sources of variability in determining malaria parasite density by microscopy.Am J Trop Med Hyg 73, no. 3 (September 2005): 593–98.
O’Meara WP, McKenzie FE, Magill AJ, Forney JR, Permpanich B, Lucas C, et al. Sources of variability in determining malaria parasite density by microscopy. Am J Trop Med Hyg. 2005 Sep;73(3):593–8.
O’Meara, Wendy Prudhomme, et al. “Sources of variability in determining malaria parasite density by microscopy.Am J Trop Med Hyg, vol. 73, no. 3, Sept. 2005, pp. 593–98.
O’Meara WP, McKenzie FE, Magill AJ, Forney JR, Permpanich B, Lucas C, Gasser RA, Wongsrichanalai C. Sources of variability in determining malaria parasite density by microscopy. Am J Trop Med Hyg. 2005 Sep;73(3):593–598.

Published In

Am J Trop Med Hyg

ISSN

0002-9637

Publication Date

September 2005

Volume

73

Issue

3

Start / End Page

593 / 598

Location

United States

Related Subject Headings

  • Tropical Medicine
  • Thailand
  • Peru
  • Observer Variation
  • Microscopy
  • Malaria, Vivax
  • Malaria, Falciparum
  • Leukocytes
  • Humans
  • Animals