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Quantification of normal cell death in the rat retina: implications for clone composition in cell lineage analysis.

Publication ,  Journal Article
Voyvodic, JT; Burne, JF; Raff, MC
Published in: Eur J Neurosci
December 1, 1995

Naturally occurring cell death complicates the analysis of cell lineage studies by making the surviving members of a clone appear more closely related than they actually are. Here we ask how much normal cell death occurs during rat retinal development, and whether that amount of death is sufficient to confuse the analysis of cell lineage relationships. We measure total cell death in the retina by combining relative counts of dead cells with absolute measurements of total cell loss. For most cell types, but not rods, we find that half of the cells generated die during normal retinal development. We use a computer model to quantify the effects of different amounts of cell death in a simulated lineage study. The simulation indicates that 50% cell death means that clonal variability analysed after the cell death period is not necessarily a good indicator of how much variability actually occurs in the underlying lineage.

Duke Scholars

Published In

Eur J Neurosci

DOI

ISSN

0953-816X

Publication Date

December 1, 1995

Volume

7

Issue

12

Start / End Page

2469 / 2478

Location

France

Related Subject Headings

  • Retina
  • Rats
  • Pregnancy
  • Neurology & Neurosurgery
  • Microscopy, Electron
  • Kinetics
  • Female
  • DNA
  • Cell Lineage
  • Cell Death
 

Citation

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Voyvodic, J. T., Burne, J. F., & Raff, M. C. (1995). Quantification of normal cell death in the rat retina: implications for clone composition in cell lineage analysis. Eur J Neurosci, 7(12), 2469–2478. https://doi.org/10.1111/j.1460-9568.1995.tb01045.x
Voyvodic, J. T., J. F. Burne, and M. C. Raff. “Quantification of normal cell death in the rat retina: implications for clone composition in cell lineage analysis.Eur J Neurosci 7, no. 12 (December 1, 1995): 2469–78. https://doi.org/10.1111/j.1460-9568.1995.tb01045.x.
Voyvodic, J. T., et al. “Quantification of normal cell death in the rat retina: implications for clone composition in cell lineage analysis.Eur J Neurosci, vol. 7, no. 12, Dec. 1995, pp. 2469–78. Pubmed, doi:10.1111/j.1460-9568.1995.tb01045.x.
Voyvodic JT, Burne JF, Raff MC. Quantification of normal cell death in the rat retina: implications for clone composition in cell lineage analysis. Eur J Neurosci. 1995 Dec 1;7(12):2469–2478.
Journal cover image

Published In

Eur J Neurosci

DOI

ISSN

0953-816X

Publication Date

December 1, 1995

Volume

7

Issue

12

Start / End Page

2469 / 2478

Location

France

Related Subject Headings

  • Retina
  • Rats
  • Pregnancy
  • Neurology & Neurosurgery
  • Microscopy, Electron
  • Kinetics
  • Female
  • DNA
  • Cell Lineage
  • Cell Death