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2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces organ- specific differential gene expression in male Japanese medaka (Oryzias latipes).

Publication ,  Journal Article
Volz, DC; Bencic, DC; Hinton, DE; Law, JM; Kullman, SW
Published in: Toxicological sciences : an official journal of the Society of Toxicology
May 2005

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a ubiquitous environmental contaminant with well-known adverse effects in fish. In this study, we initially exploited suppression subtractive hybridization (SSH) as a screening tool to assess qualitative gene expression changes in whole brain, liver, and testis of adult male Japanese medaka (Oryzias latipes) exposed for 48 h to a single intraperitoneal-injected dose of TCDD (10 microg TCDD/kg body weight). Across these three organs, SSH identified a total of 335 unique genes. Each set of forward- and reverse-subtracted organ cDNA libraries consisted of a distinct gene list and corresponding distribution of biological processes, suggesting that transcript profiles of these libraries were highly organ-specific. Based on sequence match significance and frequencies within each set of organ libraries, genes hypothesized to be strongly responsive (42 total) within male medaka brain, liver, or testis were semi-quantitatively screened with replicate cDNA nylon membrane arrays. In addition, TCDD-treated male medaka were surveyed for gross histological analysis of brain, liver, and testis. In general, adverse histopathological changes were not observed in the brain, and glycogen depletion was observed only in the liver. However, significant histological changes occurred in the testis, and included disorganization of spermatogenesis at the testis periphery, disruption of the interstitium, Leydig cell swelling, and Sertoli cell vacuolation. Of the 42 genes screened by cDNA array analysis, cytochrome P450 1A (CYP1A) mRNA was the only transcript significantly higher in TCDD-exposed brain, whereas 12 transcripts (including CYP1A) were significantly higher in TCDD-exposed liver, and 34 transcripts were significantly lower in TCDD-exposed testis. Therefore, the degree of TCDD-induced alterations observed in each organ at a gross histological level corresponded well with the number and ontology of gene transcripts affected on the array. Based on real-time reverse transcription polymerase chain reaction (RT-PCR), relative CYP1A (but not AHR1) transcript levels were confirmed to be significantly higher in TCDD-treated brain and liver. However, CYP1A was not significantly induced in TCDD-exposed testis, suggesting that gene expression and histopathological responses observed in the testis at 48 h may be CYP1A-independent. Based on these data, unique liver-specific and testis-specific mRNA-level targets in male medaka were identified as promising biomarkers of acute TCDD-induced toxicity.

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Published In

Toxicological sciences : an official journal of the Society of Toxicology

DOI

EISSN

1096-0929

ISSN

1096-6080

Publication Date

May 2005

Volume

85

Issue

1

Start / End Page

572 / 584

Related Subject Headings

  • Toxicology
  • Testis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Polychlorinated Dibenzodioxins
  • Oryzias
  • Organ Specificity
  • Oligonucleotide Array Sequence Analysis
  • Male
  • Liver
  • Injections, Intraperitoneal
 

Citation

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MLA
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Volz, D. C., Bencic, D. C., Hinton, D. E., Law, J. M., & Kullman, S. W. (2005). 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces organ- specific differential gene expression in male Japanese medaka (Oryzias latipes). Toxicological Sciences : An Official Journal of the Society of Toxicology, 85(1), 572–584. https://doi.org/10.1093/toxsci/kfi109
Volz, David C., David C. Bencic, David E. Hinton, J McHugh Law, and Seth W. Kullman. “2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces organ- specific differential gene expression in male Japanese medaka (Oryzias latipes).Toxicological Sciences : An Official Journal of the Society of Toxicology 85, no. 1 (May 2005): 572–84. https://doi.org/10.1093/toxsci/kfi109.
Volz DC, Bencic DC, Hinton DE, Law JM, Kullman SW. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces organ- specific differential gene expression in male Japanese medaka (Oryzias latipes). Toxicological sciences : an official journal of the Society of Toxicology. 2005 May;85(1):572–84.
Volz, David C., et al. “2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces organ- specific differential gene expression in male Japanese medaka (Oryzias latipes).Toxicological Sciences : An Official Journal of the Society of Toxicology, vol. 85, no. 1, May 2005, pp. 572–84. Epmc, doi:10.1093/toxsci/kfi109.
Volz DC, Bencic DC, Hinton DE, Law JM, Kullman SW. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces organ- specific differential gene expression in male Japanese medaka (Oryzias latipes). Toxicological sciences : an official journal of the Society of Toxicology. 2005 May;85(1):572–584.
Journal cover image

Published In

Toxicological sciences : an official journal of the Society of Toxicology

DOI

EISSN

1096-0929

ISSN

1096-6080

Publication Date

May 2005

Volume

85

Issue

1

Start / End Page

572 / 584

Related Subject Headings

  • Toxicology
  • Testis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Polychlorinated Dibenzodioxins
  • Oryzias
  • Organ Specificity
  • Oligonucleotide Array Sequence Analysis
  • Male
  • Liver
  • Injections, Intraperitoneal