Analysis of medaka cytochrome P450 3A homotropic and heterotropic cooperativity.
Publication
, Journal Article
Kullman, SW; Kashiwada, S; Hinton, DE
Published in: Marine environmental research
August 2004
We have previously demonstrated that medaka CYP3A is associated with metabolism of several endobiotics including steroids and bile acids. In this study, we demonstrate that medaka CYP3A catalysis exhibits unusual kinetic behaviors consistent with allosteric interaction which cannot be described by hyperbolic kinetic models. Using 7-benzyloxy-4-(trifluoromethyl)-coumarin (BFC) and nonylphenol as CYP3A substrates, we describe both homotropic and heterotropic cooperative interactions. Given the role of CYP3A in maintaining the homeostatic balance for numerous endobiotics, enzymatic activation/inhibition by endocrine disruptors (EDCs) represents a putative (non-genomic) mechanism for endocrine disruption.
Duke Scholars
Published In
Marine environmental research
DOI
EISSN
1879-0291
ISSN
0141-1136
Publication Date
August 2004
Volume
58
Issue
2-5
Start / End Page
469 / 473
Related Subject Headings
- Substrate Specificity
- Protein Binding
- Phenols
- Oxidoreductases, N-Demethylating
- Oryzias
- Models, Chemical
- Marine Biology & Hydrobiology
- Kinetics
- Fluorescence
- Cytochrome P-450 CYP3A
Citation
APA
Chicago
ICMJE
MLA
NLM
Kullman, S. W., Kashiwada, S., & Hinton, D. E. (2004). Analysis of medaka cytochrome P450 3A homotropic and heterotropic cooperativity. Marine Environmental Research, 58(2–5), 469–473. https://doi.org/10.1016/j.marenvres.2004.03.030
Kullman, Seth W., Shosaku Kashiwada, and David E. Hinton. “Analysis of medaka cytochrome P450 3A homotropic and heterotropic cooperativity.” Marine Environmental Research 58, no. 2–5 (August 2004): 469–73. https://doi.org/10.1016/j.marenvres.2004.03.030.
Kullman SW, Kashiwada S, Hinton DE. Analysis of medaka cytochrome P450 3A homotropic and heterotropic cooperativity. Marine environmental research. 2004 Aug;58(2–5):469–73.
Kullman, Seth W., et al. “Analysis of medaka cytochrome P450 3A homotropic and heterotropic cooperativity.” Marine Environmental Research, vol. 58, no. 2–5, Aug. 2004, pp. 469–73. Epmc, doi:10.1016/j.marenvres.2004.03.030.
Kullman SW, Kashiwada S, Hinton DE. Analysis of medaka cytochrome P450 3A homotropic and heterotropic cooperativity. Marine environmental research. 2004 Aug;58(2–5):469–473.
Published In
Marine environmental research
DOI
EISSN
1879-0291
ISSN
0141-1136
Publication Date
August 2004
Volume
58
Issue
2-5
Start / End Page
469 / 473
Related Subject Headings
- Substrate Specificity
- Protein Binding
- Phenols
- Oxidoreductases, N-Demethylating
- Oryzias
- Models, Chemical
- Marine Biology & Hydrobiology
- Kinetics
- Fluorescence
- Cytochrome P-450 CYP3A