Skip to main content

Microtubule disruption leads to cellular contraction in human trabecular meshwork cells.

Publication ,  Journal Article
Gills, JP; Roberts, BC; Epstein, DL
Published in: Invest Ophthalmol Vis Sci
March 1998

PURPOSE: To determine whether microtubule- and actin-altering drugs, which have been shown to increase aqueous humor outflow, cause cellular contraction in human trabecular meshwork (HTM) cells. METHODS: HTM cells were plated in culture dishes containing a polymerized deformable silicone substrate. After 48 hours, the dishes were placed on an inverted microscope and treated with ethacrynic acid, colchicine, vinblastine, cytochalasin B, or 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7) and then recorded on videotape for 15 minutes. An increase in silicone substrate wrinkle size and/or number indicated a contraction. Sham controls were used. RESULTS: Cellular contraction was observed with ethacrynic acid, colchicine, and vinblastine in the 10(-5) to 10(-4) M dosage range. Pretreatment with H-7 blocked these effects. Cytochalasin B did not produce cellular contraction. CONCLUSIONS: Microtubule disruption causes cellular contraction in HTM cells, and this effect depends on an intact actin cytoskeleton network. Contraction of trabecular meshwork cells in response to various stimuli is an attractive hypothesis for possible homeostatic mechanisms in the outflow pathway, and this may serve as a focus for novel glaucoma drug development.

Duke Scholars

Published In

Invest Ophthalmol Vis Sci

ISSN

0146-0404

Publication Date

March 1998

Volume

39

Issue

3

Start / End Page

653 / 658

Location

United States

Related Subject Headings

  • Vinblastine
  • Trabecular Meshwork
  • Ophthalmology & Optometry
  • Microtubules
  • Humans
  • Ethacrynic Acid
  • Colchicine
  • Cell Size
  • Cell Movement
  • Actins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Gills, J. P., Roberts, B. C., & Epstein, D. L. (1998). Microtubule disruption leads to cellular contraction in human trabecular meshwork cells. Invest Ophthalmol Vis Sci, 39(3), 653–658.
Gills, J. P., B. C. Roberts, and D. L. Epstein. “Microtubule disruption leads to cellular contraction in human trabecular meshwork cells.Invest Ophthalmol Vis Sci 39, no. 3 (March 1998): 653–58.
Gills JP, Roberts BC, Epstein DL. Microtubule disruption leads to cellular contraction in human trabecular meshwork cells. Invest Ophthalmol Vis Sci. 1998 Mar;39(3):653–8.
Gills, J. P., et al. “Microtubule disruption leads to cellular contraction in human trabecular meshwork cells.Invest Ophthalmol Vis Sci, vol. 39, no. 3, Mar. 1998, pp. 653–58.
Gills JP, Roberts BC, Epstein DL. Microtubule disruption leads to cellular contraction in human trabecular meshwork cells. Invest Ophthalmol Vis Sci. 1998 Mar;39(3):653–658.

Published In

Invest Ophthalmol Vis Sci

ISSN

0146-0404

Publication Date

March 1998

Volume

39

Issue

3

Start / End Page

653 / 658

Location

United States

Related Subject Headings

  • Vinblastine
  • Trabecular Meshwork
  • Ophthalmology & Optometry
  • Microtubules
  • Humans
  • Ethacrynic Acid
  • Colchicine
  • Cell Size
  • Cell Movement
  • Actins