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Viability and apoptosis of human chondrocytes in osteochondral fragments following joint trauma.

Publication ,  Journal Article
Hembree, WC; Ward, BD; Furman, BD; Zura, RD; Nichols, LA; Guilak, F; Olson, SA
Published in: J Bone Joint Surg Br
October 2007

Post-traumatic arthritis is a frequent consequence of articular fracture. The mechanisms leading to its development after such injuries have not been clearly delineated. A potential contributing factor is decreased viability of the articular chondrocytes. The object of this study was to characterise the regional variation in the viability of chondrocytes following joint trauma. A total of 29 osteochondral fragments from traumatic injuries to joints that could not be used in articular reconstruction were analysed for cell viability using the fluorescence live/dead assay and for apoptosis employing the TUNEL assay, and compared with cadaver control fragments. Chondrocyte death and apoptosis were significantly greater along the edge of the fracture and in the superficial zone of the osteochondral fragments. The middle and deep zones demonstrated significantly higher viability of the chondrocytes. These findings indicate the presence of both necrotic and apoptotic chondrocytes after joint injury and may provide further insight into the role of chondrocyte death in post-traumatic arthritis.

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Published In

J Bone Joint Surg Br

DOI

ISSN

0301-620X

Publication Date

October 2007

Volume

89

Issue

10

Start / End Page

1388 / 1395

Location

England

Related Subject Headings

  • Orthopedics
  • Middle Aged
  • Joints
  • In Situ Nick-End Labeling
  • Humans
  • Chondrocytes
  • Cell Survival
  • Cartilage, Articular
  • Arthritis
  • Apoptosis
 

Citation

APA
Chicago
ICMJE
MLA
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Hembree, W. C., Ward, B. D., Furman, B. D., Zura, R. D., Nichols, L. A., Guilak, F., & Olson, S. A. (2007). Viability and apoptosis of human chondrocytes in osteochondral fragments following joint trauma. J Bone Joint Surg Br, 89(10), 1388–1395. https://doi.org/10.1302/0301-620X.89B10.18907
Hembree, W. C., B. D. Ward, B. D. Furman, R. D. Zura, L. A. Nichols, F. Guilak, and S. A. Olson. “Viability and apoptosis of human chondrocytes in osteochondral fragments following joint trauma.J Bone Joint Surg Br 89, no. 10 (October 2007): 1388–95. https://doi.org/10.1302/0301-620X.89B10.18907.
Hembree WC, Ward BD, Furman BD, Zura RD, Nichols LA, Guilak F, et al. Viability and apoptosis of human chondrocytes in osteochondral fragments following joint trauma. J Bone Joint Surg Br. 2007 Oct;89(10):1388–95.
Hembree, W. C., et al. “Viability and apoptosis of human chondrocytes in osteochondral fragments following joint trauma.J Bone Joint Surg Br, vol. 89, no. 10, Oct. 2007, pp. 1388–95. Pubmed, doi:10.1302/0301-620X.89B10.18907.
Hembree WC, Ward BD, Furman BD, Zura RD, Nichols LA, Guilak F, Olson SA. Viability and apoptosis of human chondrocytes in osteochondral fragments following joint trauma. J Bone Joint Surg Br. 2007 Oct;89(10):1388–1395.

Published In

J Bone Joint Surg Br

DOI

ISSN

0301-620X

Publication Date

October 2007

Volume

89

Issue

10

Start / End Page

1388 / 1395

Location

England

Related Subject Headings

  • Orthopedics
  • Middle Aged
  • Joints
  • In Situ Nick-End Labeling
  • Humans
  • Chondrocytes
  • Cell Survival
  • Cartilage, Articular
  • Arthritis
  • Apoptosis