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Assembly and disassembly of DNA polymerase holoenzyme.

Publication ,  Journal Article
Sexton, DJ; Berdis, AJ; Benkovic, SJ
Published in: Curr Opin Chem Biol
October 1997

The complex task of genomic replication requires a large collection of proteins properly assembled within the close confines of the replication fork. The mechanism and dynamics of holoenzyme assembly and disassembly have been investigated using steady state and pre-steady state methods as opposed to structural studies, primarily due to the intrinsic transient nature of these protein complexes during DNA replication. The key step in bacteriophage T4 holoenzyme assembly involves ATP hydrolysis, whereas disassembly is mediated by subunit dissociation of the clamp protein in an ATP-independent manner.

Duke Scholars

Published In

Curr Opin Chem Biol

DOI

ISSN

1367-5931

Publication Date

October 1997

Volume

1

Issue

3

Start / End Page

316 / 322

Location

England

Related Subject Headings

  • Protein Processing, Post-Translational
  • Organic Chemistry
  • DNA-Directed DNA Polymerase
  • Coenzymes
  • Bacteriophage T4
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
  • 0304 Medicinal and Biomolecular Chemistry
 

Citation

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Sexton, D. J., Berdis, A. J., & Benkovic, S. J. (1997). Assembly and disassembly of DNA polymerase holoenzyme. Curr Opin Chem Biol, 1(3), 316–322. https://doi.org/10.1016/s1367-5931(97)80068-3
Sexton, D. J., A. J. Berdis, and S. J. Benkovic. “Assembly and disassembly of DNA polymerase holoenzyme.Curr Opin Chem Biol 1, no. 3 (October 1997): 316–22. https://doi.org/10.1016/s1367-5931(97)80068-3.
Sexton DJ, Berdis AJ, Benkovic SJ. Assembly and disassembly of DNA polymerase holoenzyme. Curr Opin Chem Biol. 1997 Oct;1(3):316–22.
Sexton, D. J., et al. “Assembly and disassembly of DNA polymerase holoenzyme.Curr Opin Chem Biol, vol. 1, no. 3, Oct. 1997, pp. 316–22. Pubmed, doi:10.1016/s1367-5931(97)80068-3.
Sexton DJ, Berdis AJ, Benkovic SJ. Assembly and disassembly of DNA polymerase holoenzyme. Curr Opin Chem Biol. 1997 Oct;1(3):316–322.
Journal cover image

Published In

Curr Opin Chem Biol

DOI

ISSN

1367-5931

Publication Date

October 1997

Volume

1

Issue

3

Start / End Page

316 / 322

Location

England

Related Subject Headings

  • Protein Processing, Post-Translational
  • Organic Chemistry
  • DNA-Directed DNA Polymerase
  • Coenzymes
  • Bacteriophage T4
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
  • 0304 Medicinal and Biomolecular Chemistry