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Mutations of the p16 gene product are rare in prostate cancer.

Publication ,  Journal Article
Gaddipati, JP; McLeod, DG; Sesterhenn, IA; Hussussian, CJ; Tong, YA; Seth, P; Dracopoli, NC; Moul, JW; Srivastava, S
Published in: Prostate
February 15, 1997

BACKGROUND: The p16 gene product is a negative regulator of cell cycle and has been shown to be deleted or mutated in a number of tumor cell lines and primary tumors. The role of p16 in prostate cancer is not defined. Prostate cancer tissues and cell lines were evaluated for p16 gene alterations. METHODS: Five metastatic prostate cancer cell lines were analyzed for p16 gene structure and its expression by Southern and Northern blot analyses. Forty-one DNA specimens from 18 microdissected primary tumor specimens, adjacent normal tissues, and cell lines were amplified by polymerase chain reaction for p16 protein coding and splice junction sequences. Mutations were analyzed by single strand conformation polymorphism and DNA sequencing. RESULTS: DU 145 cell line exhibited a missense mutation in codon 84 (GAC to TAC). With the exception of previously reported polymorphism, no mutation was detected in p16 coding or splice junction sequences in primary prostate cancer specimens. CONCLUSIONS: Inactivation of p16 gene by mutations in the protein coding sequence does not play a major role in the genesis of primary prostate cancer.

Duke Scholars

Published In

Prostate

DOI

ISSN

0270-4137

Publication Date

February 15, 1997

Volume

30

Issue

3

Start / End Page

188 / 194

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Prostatic Neoplasms
  • Polymorphism, Single-Stranded Conformational
  • Polymerase Chain Reaction
  • Oncology & Carcinogenesis
  • Mutation
  • Microsatellite Repeats
  • Male
  • Humans
  • Genes, Tumor Suppressor
 

Citation

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Gaddipati, J. P., McLeod, D. G., Sesterhenn, I. A., Hussussian, C. J., Tong, Y. A., Seth, P., … Srivastava, S. (1997). Mutations of the p16 gene product are rare in prostate cancer. Prostate, 30(3), 188–194. https://doi.org/10.1002/(sici)1097-0045(19970215)30:3<188::aid-pros7>3.0.co;2-i
Gaddipati, J. P., D. G. McLeod, I. A. Sesterhenn, C. J. Hussussian, Y. A. Tong, P. Seth, N. C. Dracopoli, J. W. Moul, and S. Srivastava. “Mutations of the p16 gene product are rare in prostate cancer.Prostate 30, no. 3 (February 15, 1997): 188–94. https://doi.org/10.1002/(sici)1097-0045(19970215)30:3<188::aid-pros7>3.0.co;2-i.
Gaddipati JP, McLeod DG, Sesterhenn IA, Hussussian CJ, Tong YA, Seth P, et al. Mutations of the p16 gene product are rare in prostate cancer. Prostate. 1997 Feb 15;30(3):188–94.
Gaddipati, J. P., et al. “Mutations of the p16 gene product are rare in prostate cancer.Prostate, vol. 30, no. 3, Feb. 1997, pp. 188–94. Pubmed, doi:10.1002/(sici)1097-0045(19970215)30:3<188::aid-pros7>3.0.co;2-i.
Gaddipati JP, McLeod DG, Sesterhenn IA, Hussussian CJ, Tong YA, Seth P, Dracopoli NC, Moul JW, Srivastava S. Mutations of the p16 gene product are rare in prostate cancer. Prostate. 1997 Feb 15;30(3):188–194.
Journal cover image

Published In

Prostate

DOI

ISSN

0270-4137

Publication Date

February 15, 1997

Volume

30

Issue

3

Start / End Page

188 / 194

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Prostatic Neoplasms
  • Polymorphism, Single-Stranded Conformational
  • Polymerase Chain Reaction
  • Oncology & Carcinogenesis
  • Mutation
  • Microsatellite Repeats
  • Male
  • Humans
  • Genes, Tumor Suppressor