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Methylation of TFPI-2 gene is not the sole cause of its silencing.

Publication ,  Journal Article
Rao, CN; Segawa, T; Navari, JR; Xu, L; Srivastava, S; Moul, JW; Phillips, B
Published in: International journal of oncology
April 2003

TFPI-2 (tissue factor pathway inhibitor-2) is a serine protease inhibitor that may suppress tumor cell invasion and metastasis. TFPI-2 expression is often lost in cells derived from tumors of diverse organs. We have examined whether aberrant hypermethylation of the 5' end of the TFPI-2 gene is associated with its loss of expression. After 5-azacytidine treatment of three cell lines lacking TFPI-2 expression (HT1080 fibrosarcoma cells, MCF-7 breast carcinoma cells, and LNCaP prostate carcinoma cells), TFPI-2 transcripts could be detected by RT-PCR. In these three cell lines, methylation of the 5' end of the TFPI-2 gene was detected, while two prostate carcinoma cell lines in which the TFPI-2 gene was expressed, PC-3 and DU-145, showed no methylation. However, all the three cell lines which lacked TFPI-2 expression also contained unmethylated TFPI-2 alleles. Furthermore, a transiently transfected TFPI-2 promoter was non-functional in the three cell lines, but function was attained following treatment with 5-azacytidine. Our results indicate that while methylation of the TFPI-2 gene is associated with its silencing, it is not the sole cause, and we suggest that one or more components of pathways regulating TFPI-2 expression have also undergone methylation-associated silencing in these cell lines.

Duke Scholars

Published In

International journal of oncology

EISSN

1791-2423

ISSN

1019-6439

Publication Date

April 2003

Volume

22

Issue

4

Start / End Page

843 / 848

Related Subject Headings

  • Transfection
  • Sulfites
  • Reverse Transcriptase Polymerase Chain Reaction
  • Oncology & Carcinogenesis
  • Models, Genetic
  • Male
  • Luciferases
  • Humans
  • Glycoproteins
  • Genes, Reporter
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Rao, C. N., Segawa, T., Navari, J. R., Xu, L., Srivastava, S., Moul, J. W., & Phillips, B. (2003). Methylation of TFPI-2 gene is not the sole cause of its silencing. International Journal of Oncology, 22(4), 843–848.
Rao, Chilukuri N., Takehiko Segawa, Jason R. Navari, Linda Xu, Shiv Srivastava, Judd W. Moul, and Benette Phillips. “Methylation of TFPI-2 gene is not the sole cause of its silencing.International Journal of Oncology 22, no. 4 (April 2003): 843–48.
Rao CN, Segawa T, Navari JR, Xu L, Srivastava S, Moul JW, et al. Methylation of TFPI-2 gene is not the sole cause of its silencing. International journal of oncology. 2003 Apr;22(4):843–8.
Rao, Chilukuri N., et al. “Methylation of TFPI-2 gene is not the sole cause of its silencing.International Journal of Oncology, vol. 22, no. 4, Apr. 2003, pp. 843–48.
Rao CN, Segawa T, Navari JR, Xu L, Srivastava S, Moul JW, Phillips B. Methylation of TFPI-2 gene is not the sole cause of its silencing. International journal of oncology. 2003 Apr;22(4):843–848.

Published In

International journal of oncology

EISSN

1791-2423

ISSN

1019-6439

Publication Date

April 2003

Volume

22

Issue

4

Start / End Page

843 / 848

Related Subject Headings

  • Transfection
  • Sulfites
  • Reverse Transcriptase Polymerase Chain Reaction
  • Oncology & Carcinogenesis
  • Models, Genetic
  • Male
  • Luciferases
  • Humans
  • Glycoproteins
  • Genes, Reporter