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Nitric oxide synthase from cerebellum catalyzes the formation of equimolar quantities of nitric oxide and citrulline from L-arginine.

Publication ,  Journal Article
Bush, PA; Gonzalez, NE; Griscavage, JM; Ignarro, LJ
Published in: Biochemical and biophysical research communications
June 1992

This study examined whether constitutive nitric oxide (NO) synthase from rat cerebellum catalyzes the formation of equimolar amounts of NO plus citrulline from L-arginine under various conditions. Citrulline was determined by monitoring the formation of 3H-citrulline from 3H-L-arginine. NO was determined by monitoring the formation of total NOx (NO+nitrite [NO2-] + nitrate [NO3-]) by chemiluminescence after reduction of NOx to NO by acidic vanadium (III). Equal quantities of NO plus citrulline were generated from L-arginine and the formation of both products was linear for about 20 min at 37 degrees C provided L-arginine was present in excess to maintain a zero order reaction rate. Deletion of NADPH, addition of the calmodulin antagonist calmidazolium, or addition of NO synthase inhibitors (NG-methyl-L-arginine, NG-amino-L-arginine) abolished or markedly inhibited the formation of both NO and citrulline. The Km for L-arginine (14 microM; 18 microM) and the Vmax of the reaction (0.74 nmol/min/mg protein; 0.67 nmol/min/mg protein) were the same whether NO or citrulline formation, respectively, was monitored. These observations indicate clearly that NO and citrulline are formed in equimolar quantities from L-arginine by the constitutive isoform of NO synthase from rat cerebellum.

Duke Scholars

Published In

Biochemical and biophysical research communications

ISSN

0006-291X

Publication Date

June 1992

Volume

185

Issue

3

Start / End Page

960 / 966

Related Subject Headings

  • Tritium
  • Rats, Inbred Strains
  • Rats
  • Radioisotope Dilution Technique
  • Nitric Oxide Synthase
  • Nitric Oxide
  • Male
  • Luminescent Measurements
  • Kinetics
  • Citrulline
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Bush, P. A., Gonzalez, N. E., Griscavage, J. M., & Ignarro, L. J. (1992). Nitric oxide synthase from cerebellum catalyzes the formation of equimolar quantities of nitric oxide and citrulline from L-arginine. Biochemical and Biophysical Research Communications, 185(3), 960–966.
Bush, P. A., N. E. Gonzalez, J. M. Griscavage, and L. J. Ignarro. “Nitric oxide synthase from cerebellum catalyzes the formation of equimolar quantities of nitric oxide and citrulline from L-arginine.Biochemical and Biophysical Research Communications 185, no. 3 (June 1992): 960–66.
Bush PA, Gonzalez NE, Griscavage JM, Ignarro LJ. Nitric oxide synthase from cerebellum catalyzes the formation of equimolar quantities of nitric oxide and citrulline from L-arginine. Biochemical and biophysical research communications. 1992 Jun;185(3):960–6.
Bush, P. A., et al. “Nitric oxide synthase from cerebellum catalyzes the formation of equimolar quantities of nitric oxide and citrulline from L-arginine.Biochemical and Biophysical Research Communications, vol. 185, no. 3, June 1992, pp. 960–66.
Bush PA, Gonzalez NE, Griscavage JM, Ignarro LJ. Nitric oxide synthase from cerebellum catalyzes the formation of equimolar quantities of nitric oxide and citrulline from L-arginine. Biochemical and biophysical research communications. 1992 Jun;185(3):960–966.
Journal cover image

Published In

Biochemical and biophysical research communications

ISSN

0006-291X

Publication Date

June 1992

Volume

185

Issue

3

Start / End Page

960 / 966

Related Subject Headings

  • Tritium
  • Rats, Inbred Strains
  • Rats
  • Radioisotope Dilution Technique
  • Nitric Oxide Synthase
  • Nitric Oxide
  • Male
  • Luminescent Measurements
  • Kinetics
  • Citrulline