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Biology of hepatocellular neoplasia in the mouse. III. Electron microscopy of safrole-induced hepatocellular adenomas and hepatocellular carcinomas.

Publication ,  Journal Article
Lipsky, MM; Hinton, DE; Klaunig, JE; Trump, BF
Published in: Journal of the National Cancer Institute
August 1981

A systematic, ultrastructural analysis wsa performed on safrole-induced hepatocellular adenomas and hepatocellular carcinoma(s) (HPC) in BALB/c mice. Adenomas were heterogeneous in cell composition containing dark-staining basophilic cells, pale-staining acidophilic cells, clear cells, and lipid-laden cells. Darkly staining cells resembled fetal hepatocytes. They had large nuclei with irregular borders and limited diversity of organelles. Rough endoplasmic reticulum was prominent and seen as parallel cisternae in single or double tracts often in association with mitochondria. Pale-staining cells contained abundant smooth endoplasmic reticulum. Other organelles were often displaced to the perinuclear or peripheral region of the cell. The clear cells resembled dark-staining or pale-staining cells but also containing large areas of glycogen deposition. Lipid-laden cells contained numerous, multisized lipid droplets in the cytoplasm. HPC contained cell types similar to those of the adenoma. In addition, they contained many anaplastic cells. These resembled hepatocytes but contained several other alterations. The most striking was an apparent increase in the number of altered mitochondria. The cytoplasm was often fluid with enlarged mitochondria with dense or pale matrices. The cristae were few and had altered configurations. Also, an apparent increase was seen in the number of microbodies. These were often clustered in one region of the cytoplasm. An increase in microbodies was also noted in other cell types of hepatocellular carcinomas. The results of this study demonstrated similarities in the cell types of the adenomas and HPC. This study also demonstrated differences, with the anaplastic cell being common only to the carcinoma. Due to the similarity of cell types, the adenoma should be considered a possible site of HPC development.

Duke Scholars

Published In

Journal of the National Cancer Institute

EISSN

1460-2105

ISSN

0027-8874

Publication Date

August 1981

Volume

67

Issue

2

Start / End Page

393 / 405

Related Subject Headings

  • Safrole
  • Oncology & Carcinogenesis
  • Mitochondria, Liver
  • Microscopy, Electron
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Liver Neoplasms, Experimental
  • Endoplasmic Reticulum
  • Dioxoles
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lipsky, M. M., Hinton, D. E., Klaunig, J. E., & Trump, B. F. (1981). Biology of hepatocellular neoplasia in the mouse. III. Electron microscopy of safrole-induced hepatocellular adenomas and hepatocellular carcinomas. Journal of the National Cancer Institute, 67(2), 393–405.
Lipsky, M. M., D. E. Hinton, J. E. Klaunig, and B. F. Trump. “Biology of hepatocellular neoplasia in the mouse. III. Electron microscopy of safrole-induced hepatocellular adenomas and hepatocellular carcinomas.Journal of the National Cancer Institute 67, no. 2 (August 1981): 393–405.
Lipsky MM, Hinton DE, Klaunig JE, Trump BF. Biology of hepatocellular neoplasia in the mouse. III. Electron microscopy of safrole-induced hepatocellular adenomas and hepatocellular carcinomas. Journal of the National Cancer Institute. 1981 Aug;67(2):393–405.
Lipsky, M. M., et al. “Biology of hepatocellular neoplasia in the mouse. III. Electron microscopy of safrole-induced hepatocellular adenomas and hepatocellular carcinomas.Journal of the National Cancer Institute, vol. 67, no. 2, Aug. 1981, pp. 393–405.
Lipsky MM, Hinton DE, Klaunig JE, Trump BF. Biology of hepatocellular neoplasia in the mouse. III. Electron microscopy of safrole-induced hepatocellular adenomas and hepatocellular carcinomas. Journal of the National Cancer Institute. 1981 Aug;67(2):393–405.
Journal cover image

Published In

Journal of the National Cancer Institute

EISSN

1460-2105

ISSN

0027-8874

Publication Date

August 1981

Volume

67

Issue

2

Start / End Page

393 / 405

Related Subject Headings

  • Safrole
  • Oncology & Carcinogenesis
  • Mitochondria, Liver
  • Microscopy, Electron
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Liver Neoplasms, Experimental
  • Endoplasmic Reticulum
  • Dioxoles