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A possible role for potassium and pyruvate in the modulation of sperm motility in the rabbit oviducal isthmus.

Publication ,  Journal Article
Burkman, LJ; Overstreet, JW; Katz, DF
Published in: Journal of Reproduction and Fertility
July 1984

Spermatozoa were recovered from the isthmus of the rabbit oviduct at 4 and 11 h post coitum using several defined flushing media. The motility of spermatozoa in the isthmic flushings was subsequently analysed from video recordings. There was little sperm movement in the native isthmic fluid, but vigorous flagellar activity and hyperactivated movement were induced by flushing the isthmus with 0.25 M-sucrose, apparently an effect of dilution. Flushing with a complex culture medium resulted in similar stimulation of sperm movement. When pyruvate was present in the medium, hyperactivated flagellar bending was stimulated, whereas these movements were virtually absent when glucose alone was present. The stimulating effect of dilution was less pronounced when the flushing medium contained 50 mM-potassium. When the isthmus was flushed with media containing 50 mM-K+ and the K+ concentration was lowered to 5 mM during a washing procedure, large-amplitude flagellar movements were sequentially suppressed and restored. The restoration of large-amplitude movements was enhanced when pyruvate was present in the 5 mM-K+ washing medium. These results suggest that alterations in the concentration of both K+ and pyruvate may have a role in regulating the motility of rabbit spermatozoa in the oviducal isthmus, K+ being inhibitory and pyruvate stimulatory.

Duke Scholars

Published In

Journal of Reproduction and Fertility

DOI

ISSN

0022-4251

Publication Date

July 1984

Volume

71

Issue

2

Start / End Page

367 / 376

Related Subject Headings

  • Sperm Transport
  • Sperm Motility
  • Rabbits
  • Pyruvic Acid
  • Pyruvates
  • Potassium
  • Obstetrics & Reproductive Medicine
  • Male
  • Female
  • Fallopian Tubes
 

Citation

APA
Chicago
ICMJE
MLA
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Burkman, L. J., Overstreet, J. W., & Katz, D. F. (1984). A possible role for potassium and pyruvate in the modulation of sperm motility in the rabbit oviducal isthmus. Journal of Reproduction and Fertility, 71(2), 367–376. https://doi.org/10.1530/jrf.0.0710367
Burkman, L. J., J. W. Overstreet, and D. F. Katz. “A possible role for potassium and pyruvate in the modulation of sperm motility in the rabbit oviducal isthmus.Journal of Reproduction and Fertility 71, no. 2 (July 1984): 367–76. https://doi.org/10.1530/jrf.0.0710367.
Burkman LJ, Overstreet JW, Katz DF. A possible role for potassium and pyruvate in the modulation of sperm motility in the rabbit oviducal isthmus. Journal of Reproduction and Fertility. 1984 Jul;71(2):367–76.
Burkman, L. J., et al. “A possible role for potassium and pyruvate in the modulation of sperm motility in the rabbit oviducal isthmus.Journal of Reproduction and Fertility, vol. 71, no. 2, July 1984, pp. 367–76. Epmc, doi:10.1530/jrf.0.0710367.
Burkman LJ, Overstreet JW, Katz DF. A possible role for potassium and pyruvate in the modulation of sperm motility in the rabbit oviducal isthmus. Journal of Reproduction and Fertility. 1984 Jul;71(2):367–376.

Published In

Journal of Reproduction and Fertility

DOI

ISSN

0022-4251

Publication Date

July 1984

Volume

71

Issue

2

Start / End Page

367 / 376

Related Subject Headings

  • Sperm Transport
  • Sperm Motility
  • Rabbits
  • Pyruvic Acid
  • Pyruvates
  • Potassium
  • Obstetrics & Reproductive Medicine
  • Male
  • Female
  • Fallopian Tubes