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Escherichia coli mutY gene encodes an adenine glycosylase active on G-A mispairs.

Publication ,  Journal Article
Au, KG; Clark, S; Miller, JH; Modrich, P
Published in: Proc Natl Acad Sci U S A
November 1989

Mutations in the mutY gene of Escherichia coli confer hypermutability reflecting G.C to T.A transversion mutations and result in a deficiency in methyl-independent G-A to G.C mismatch correction. In the present work, the mutY product has been purified to near homogeneity by virtue of its ability to restore G-A to G.C mismatch correction to cell-free extracts of a mutS mutY strain. The 36-kDa protein renders the strand containing the mispaired adenine labile to base-catalyzed cleavage and sensitive to cleavage by several apurinic/apyrimidinic-site endonucleases, with the sites of strand scission by both agents corresponding to the location of the mismatch. These findings indicate that MutY is a DNA glycosylase that hydrolyzes the glycosyl bond linking the mis-paired adenine to deoxyribose. MutY, a 5'-apurinic/apyrimidinic-site endonuclease, DNA polymerase I, and DNA ligase are sufficient to reconstitute MutY-dependent G-A to G.C repair in vitro.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

November 1989

Volume

86

Issue

22

Start / End Page

8877 / 8881

Location

United States

Related Subject Headings

  • N-Glycosyl Hydrolases
  • Guanine
  • Genes, Bacterial
  • Escherichia coli
  • DNA, Bacterial
  • DNA Repair
  • DNA Glycosylases
  • Base Composition
  • Adenine
 

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Au, K. G., Clark, S., Miller, J. H., & Modrich, P. (1989). Escherichia coli mutY gene encodes an adenine glycosylase active on G-A mispairs. Proc Natl Acad Sci U S A, 86(22), 8877–8881. https://doi.org/10.1073/pnas.86.22.8877
Au, K. G., S. Clark, J. H. Miller, and P. Modrich. “Escherichia coli mutY gene encodes an adenine glycosylase active on G-A mispairs.Proc Natl Acad Sci U S A 86, no. 22 (November 1989): 8877–81. https://doi.org/10.1073/pnas.86.22.8877.
Au KG, Clark S, Miller JH, Modrich P. Escherichia coli mutY gene encodes an adenine glycosylase active on G-A mispairs. Proc Natl Acad Sci U S A. 1989 Nov;86(22):8877–81.
Au, K. G., et al. “Escherichia coli mutY gene encodes an adenine glycosylase active on G-A mispairs.Proc Natl Acad Sci U S A, vol. 86, no. 22, Nov. 1989, pp. 8877–81. Pubmed, doi:10.1073/pnas.86.22.8877.
Au KG, Clark S, Miller JH, Modrich P. Escherichia coli mutY gene encodes an adenine glycosylase active on G-A mispairs. Proc Natl Acad Sci U S A. 1989 Nov;86(22):8877–8881.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

November 1989

Volume

86

Issue

22

Start / End Page

8877 / 8881

Location

United States

Related Subject Headings

  • N-Glycosyl Hydrolases
  • Guanine
  • Genes, Bacterial
  • Escherichia coli
  • DNA, Bacterial
  • DNA Repair
  • DNA Glycosylases
  • Base Composition
  • Adenine