Investigation of the complexes of EcoRI endonuclease with decanucleotides containing canonical and modified recognition sequences using fluorescence and optical detection of magnetic resonance spectroscopy.
Publication
, Journal Article
Jhon, NI; Casas-Finet, JR; Maki, AH; Modrich, P
Published in: Biochim Biophys Acta
February 28, 1988
The binding of EcoRI endonuclease to the oligonucleotides d(GCGAATTCGC) and d(GCGAA) (5BrdU) (5BrdU) d(CGC) has been investigated to determine whether stacking interactions occur between tryptophan residues and the DNA bases. Fluorescence binding isotherms show that the decamer containing the canonical and that containing the modified recognition sequence bind with comparable affinity. Optically detected magnetic resonance spectra show limited perturbations of the Trp zero-field splitting parameters, which are assigned to electrical field effects. No evidence for Trp stacking interactions has been found.
Duke Scholars
Published In
Biochim Biophys Acta
DOI
ISSN
0006-3002
Publication Date
February 28, 1988
Volume
949
Issue
2
Start / End Page
189 / 194
Location
Netherlands
Related Subject Headings
- Tryptophan
- Spectrometry, Fluorescence
- Protein Binding
- Magnetic Resonance Spectroscopy
- In Vitro Techniques
- Deoxyribonuclease EcoRI
- DNA-Binding Proteins
- DNA Restriction Enzymes
- DNA
- Binding Sites
Citation
APA
Chicago
ICMJE
MLA
NLM
Jhon, N. I., Casas-Finet, J. R., Maki, A. H., & Modrich, P. (1988). Investigation of the complexes of EcoRI endonuclease with decanucleotides containing canonical and modified recognition sequences using fluorescence and optical detection of magnetic resonance spectroscopy. Biochim Biophys Acta, 949(2), 189–194. https://doi.org/10.1016/0167-4781(88)90082-6
Jhon, N. I., J. R. Casas-Finet, A. H. Maki, and P. Modrich. “Investigation of the complexes of EcoRI endonuclease with decanucleotides containing canonical and modified recognition sequences using fluorescence and optical detection of magnetic resonance spectroscopy.” Biochim Biophys Acta 949, no. 2 (February 28, 1988): 189–94. https://doi.org/10.1016/0167-4781(88)90082-6.
Jhon NI, Casas-Finet JR, Maki AH, Modrich P. Investigation of the complexes of EcoRI endonuclease with decanucleotides containing canonical and modified recognition sequences using fluorescence and optical detection of magnetic resonance spectroscopy. Biochim Biophys Acta. 1988 Feb 28;949(2):189–94.
Jhon, N. I., et al. “Investigation of the complexes of EcoRI endonuclease with decanucleotides containing canonical and modified recognition sequences using fluorescence and optical detection of magnetic resonance spectroscopy.” Biochim Biophys Acta, vol. 949, no. 2, Feb. 1988, pp. 189–94. Pubmed, doi:10.1016/0167-4781(88)90082-6.
Jhon NI, Casas-Finet JR, Maki AH, Modrich P. Investigation of the complexes of EcoRI endonuclease with decanucleotides containing canonical and modified recognition sequences using fluorescence and optical detection of magnetic resonance spectroscopy. Biochim Biophys Acta. 1988 Feb 28;949(2):189–194.
Published In
Biochim Biophys Acta
DOI
ISSN
0006-3002
Publication Date
February 28, 1988
Volume
949
Issue
2
Start / End Page
189 / 194
Location
Netherlands
Related Subject Headings
- Tryptophan
- Spectrometry, Fluorescence
- Protein Binding
- Magnetic Resonance Spectroscopy
- In Vitro Techniques
- Deoxyribonuclease EcoRI
- DNA-Binding Proteins
- DNA Restriction Enzymes
- DNA
- Binding Sites