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Genetic mapping of Ty elements in Saccharomyces cerevisiae.

Publication ,  Journal Article
Klein, HL; Petes, TD
Published in: Mol Cell Biol
February 1984

We used transformation to insert a selectable marker at various sites in the Saccharomyces cerevisiae genome occupied by the transposable element Ty. The vector CV9 contains the LEU2+ gene and a portion of the repeated element Ty1-17. Transformation with this plasmid resulted in integration of the vector via a reciprocal exchange using homology at the LEU2 locus or at the various Ty elements that are dispersed throughout the S. cerevisiae genome. These transformants were used to map genetically sites of several Ty elements. The 24 transformants recovered at Ty sites define 19 distinct loci. Seven of these were placed on the genetic map. Two classes of Ty elements were identified in these experiments: a Ty1-17 class and Ty elements different from Ty1-17. Statistical analysis of the number of transformants at each class of Ty elements shows that there is preferential integration of the CV9 plasmid into the Ty1-17 class.

Duke Scholars

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

February 1984

Volume

4

Issue

2

Start / End Page

329 / 339

Location

United States

Related Subject Headings

  • Transformation, Genetic
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Developmental Biology
  • DNA, Fungal
  • Culture Media
  • Chromosome Mapping
  • Centromere
  • 42 Health sciences
  • 32 Biomedical and clinical sciences
 

Citation

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Klein, H. L., & Petes, T. D. (1984). Genetic mapping of Ty elements in Saccharomyces cerevisiae. Mol Cell Biol, 4(2), 329–339. https://doi.org/10.1128/mcb.4.2.329-339.1984
Klein, H. L., and T. D. Petes. “Genetic mapping of Ty elements in Saccharomyces cerevisiae.Mol Cell Biol 4, no. 2 (February 1984): 329–39. https://doi.org/10.1128/mcb.4.2.329-339.1984.
Klein HL, Petes TD. Genetic mapping of Ty elements in Saccharomyces cerevisiae. Mol Cell Biol. 1984 Feb;4(2):329–39.
Klein, H. L., and T. D. Petes. “Genetic mapping of Ty elements in Saccharomyces cerevisiae.Mol Cell Biol, vol. 4, no. 2, Feb. 1984, pp. 329–39. Pubmed, doi:10.1128/mcb.4.2.329-339.1984.
Klein HL, Petes TD. Genetic mapping of Ty elements in Saccharomyces cerevisiae. Mol Cell Biol. 1984 Feb;4(2):329–339.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

February 1984

Volume

4

Issue

2

Start / End Page

329 / 339

Location

United States

Related Subject Headings

  • Transformation, Genetic
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Developmental Biology
  • DNA, Fungal
  • Culture Media
  • Chromosome Mapping
  • Centromere
  • 42 Health sciences
  • 32 Biomedical and clinical sciences