EXCITATION OF FLUORESCENT EMISSION FROM SOLUTIONS AT THE SURFACE OF POLYMER THIN-FILM WAVEGUIDES: AN INTEGRATED OPTICS TECHNIQUE FOR THE SENSING OF FLUORESCENCE AT THE POLYMER/SOLUTION INTERFACE.

The authors report a technique for the excitation and collection of fluorescence in a flow cell coupled to the surface of an integrated optical waveguide. The waveguides were 1-2- mu m-thick poly(styrene) films spun-cast onto acid-cleaned Pyrex microscope slides. The flow cell was used to introduce fluorescent solutions to the light energy at the polymer waveguide surface. Fluorescence data from the waveguide surface excitation of fluorescein solutions are presented. The application of polymer thin-film waveguides to the study of protein adsorption at the polymer/solution interface is discussed.

Duke Scholars

Author William M. Reichert Biomedical Engineering