
Evaluation of antibody immobilization technqiues for fiber optic-based fluoroimmunosensing
Fiber optic chemical sensors have been developed that employ immunochemicals to perform fluoroimmunoassays. A regenerable fiber optic sensor was developed with a capillary delivery system to perform repetitive assays using antibodies immobilized to beads, "immunobeads". The sensitivity of these sensors is directly proportional to the amount of antibody present. For the immunobeads, the amount immobilized (loaded) and the ability of the immobilized antibody to maintain antibody recognition (activity) are two criteria which will affect the sensitivity of the sensors. Four reagents, [1,1′-carbonyldiimidazole (CDI), Protein A, glycidoxypropyltrimethoxysilane (GOPS) and 2-fluoro-1-methylpyridium toluene-4-sulfonate (FMP)], were evaluated using these two criteria. Two antibody-antigen systems were employed to investigate the four procedures. The first combination is a polyclonal rabbit anti-human IgG with a F(ab)′
Duke Scholars
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- Analytical Chemistry
- 4018 Nanotechnology
- 4004 Chemical engineering
- 3401 Analytical chemistry
- 0399 Other Chemical Sciences
- 0301 Analytical Chemistry
Citation

Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Related Subject Headings
- Analytical Chemistry
- 4018 Nanotechnology
- 4004 Chemical engineering
- 3401 Analytical chemistry
- 0399 Other Chemical Sciences
- 0301 Analytical Chemistry