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Extended storage of AS-1 and AS-3 leukoreduced red blood cells for 15 days after deglycerolization and resuspension in AS-3 using an automated closed system.

Publication ,  Journal Article
Bandarenko, N; Hay, SN; Holmberg, J; Whitley, P; Taylor, HL; Moroff, G; Rose, L; Kowalsky, R; Brumit, M; Rose, M; Sawyer, S; Johnson, A ...
Published in: Transfusion
November 2004

BACKGROUND: The utilization of cryopreserved red blood cell (RBC) units had been limited by a maximum postdeglycerolization storage of 24 hours at 1 to 6 degrees C until the recent development of a closed system for the glycerolization and deglycerolization process. STUDY DESIGN AND METHODS: Sixty leukoreduced additive solution (AS), AS-1 (n = 30) and AS-3 (n = 30) RBC units from 500-mL whole blood (WB) collections were stored for 6 days, glycerolized, frozen at -70 +/- 5 degrees C for at least 14 days, thawed, deglycerolized, and stored for 15 days at 1 to 6 degrees C. Glycerolization and deglycerolization were performed with the ACP 215. In-vitro variables were tested before glycerolization, on Day 0, and Day 15 after deglycerolization storage. Forty donors were assessed for double-label 24-hour percent recovery, and T1/2 survival time was measured for 20 donors. RESULTS: Postdeglycerolization mean +/- standard deviation in-vitro RBC mass recoveries were 93 +/- 5 percent for AS-1 and 95 +/- 4 percent for AS-3. Mean hemoglobin +/- standard deviation after deglycerolization was 50.5 +/- 5.5g for AS-1 and 50.1 +/- 3.5g for AS-3. Mean hemolysis (Day 15) was 0.36 +/- 0.11 percent for AS-1 and 0.38 +/- 0.13 percent for AS-3. Double-label 24-hour in-vivo recoveries were 82.5 +/- 7.8 percent for AS-1 and 81.4 +/- 7.1 percent for AS-3. The 51Cr T1/2 value was 41.8 +/- 3.97 for AS-1 and 40.6 +/- 7.11 for AS-3. Other in-vitro variables were as expected. CONCLUSION: Leukoreduced AS-1 and AS-3 RBCs after frozen storage at -70 +/- 5 degrees C can be stored for up to 14 days when processing is performed with the ACP 215 system with resuspension of deglycerolized RBCs in AS-3.

Duke Scholars

Published In

Transfusion

DOI

ISSN

0041-1132

Publication Date

November 2004

Volume

44

Issue

11

Start / End Page

1656 / 1662

Location

United States

Related Subject Headings

  • Time Factors
  • Sodium Chloride
  • Phosphates
  • Mannitol
  • Leukocytes
  • Humans
  • Glycerol
  • Glucose
  • Erythrocytes
  • Erythrocyte Transfusion
 

Citation

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Chicago
ICMJE
MLA
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Bandarenko, N., Hay, S. N., Holmberg, J., Whitley, P., Taylor, H. L., Moroff, G., … Popovsky, M. A. (2004). Extended storage of AS-1 and AS-3 leukoreduced red blood cells for 15 days after deglycerolization and resuspension in AS-3 using an automated closed system. Transfusion, 44(11), 1656–1662. https://doi.org/10.1111/j.1537-2995.2004.04101.x
Bandarenko, Nicholas, Shauna N. Hay, Jerry Holmberg, Pam Whitley, Harry L. Taylor, Gary Moroff, Leslie Rose, et al. “Extended storage of AS-1 and AS-3 leukoreduced red blood cells for 15 days after deglycerolization and resuspension in AS-3 using an automated closed system.Transfusion 44, no. 11 (November 2004): 1656–62. https://doi.org/10.1111/j.1537-2995.2004.04101.x.
Bandarenko N, Hay SN, Holmberg J, Whitley P, Taylor HL, Moroff G, et al. Extended storage of AS-1 and AS-3 leukoreduced red blood cells for 15 days after deglycerolization and resuspension in AS-3 using an automated closed system. Transfusion. 2004 Nov;44(11):1656–62.
Bandarenko, Nicholas, et al. “Extended storage of AS-1 and AS-3 leukoreduced red blood cells for 15 days after deglycerolization and resuspension in AS-3 using an automated closed system.Transfusion, vol. 44, no. 11, Nov. 2004, pp. 1656–62. Pubmed, doi:10.1111/j.1537-2995.2004.04101.x.
Bandarenko N, Hay SN, Holmberg J, Whitley P, Taylor HL, Moroff G, Rose L, Kowalsky R, Brumit M, Rose M, Sawyer S, Johnson A, McNeil D, Popovsky MA. Extended storage of AS-1 and AS-3 leukoreduced red blood cells for 15 days after deglycerolization and resuspension in AS-3 using an automated closed system. Transfusion. 2004 Nov;44(11):1656–1662.
Journal cover image

Published In

Transfusion

DOI

ISSN

0041-1132

Publication Date

November 2004

Volume

44

Issue

11

Start / End Page

1656 / 1662

Location

United States

Related Subject Headings

  • Time Factors
  • Sodium Chloride
  • Phosphates
  • Mannitol
  • Leukocytes
  • Humans
  • Glycerol
  • Glucose
  • Erythrocytes
  • Erythrocyte Transfusion