Visualizing receptor endocytosis and trafficking.
Publication
, Journal Article
Salahpour, A; Barak, LS
Published in: Methods Mol Biol
2011
G-protein-coupled 7 transmembrane domain receptors (GPCR-7TMR) represent the largest class of membrane protein drug targets. They respond to a plethora of ligands ranging from small molecules to polypeptide hormones. Upon activation, almost all GPCR-7TMRs undergo desensitization followed by receptor internalization and resensitization. This cycle is crucially important for regulating the signal emanating from the receptor and is tightly linked to the receptor and/or the ligands studied. In this chapter, we describe some of the technical approaches that can be used to study GPCR internalization and trafficking.
Duke Scholars
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2011
Volume
756
Start / End Page
311 / 323
Location
United States
Related Subject Headings
- Transfection
- Receptors, G-Protein-Coupled
- Protein Transport
- Microscopy, Confocal
- Humans
- Green Fluorescent Proteins
- Gene Expression
- Fluorescent Antibody Technique
- Endocytosis
- Developmental Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Salahpour, A., & Barak, L. S. (2011). Visualizing receptor endocytosis and trafficking. Methods Mol Biol, 756, 311–323. https://doi.org/10.1007/978-1-61779-160-4_18
Salahpour, Ali, and Larry S. Barak. “Visualizing receptor endocytosis and trafficking.” Methods Mol Biol 756 (2011): 311–23. https://doi.org/10.1007/978-1-61779-160-4_18.
Salahpour A, Barak LS. Visualizing receptor endocytosis and trafficking. Methods Mol Biol. 2011;756:311–23.
Salahpour, Ali, and Larry S. Barak. “Visualizing receptor endocytosis and trafficking.” Methods Mol Biol, vol. 756, 2011, pp. 311–23. Pubmed, doi:10.1007/978-1-61779-160-4_18.
Salahpour A, Barak LS. Visualizing receptor endocytosis and trafficking. Methods Mol Biol. 2011;756:311–323.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2011
Volume
756
Start / End Page
311 / 323
Location
United States
Related Subject Headings
- Transfection
- Receptors, G-Protein-Coupled
- Protein Transport
- Microscopy, Confocal
- Humans
- Green Fluorescent Proteins
- Gene Expression
- Fluorescent Antibody Technique
- Endocytosis
- Developmental Biology